Addgene

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TALED_Right-stuffer-8e

Plasmid

#187165

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-E1347A

Plasmid

#187164

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Left-stuffer-8e

Plasmid

#187162

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

stuffer-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-E1347A

Plasmid

#187161

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-1397N-UGI

Plasmid

#187174

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397N-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397C-UGI

Plasmid

#187171

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397C-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

Plasmid

#187273

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Anillin A740D, E758K

Promoter

U6, CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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Cdk3

Plasmid

#1878

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk3 (CDK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
Cdk3-DN

Plasmid

#1879

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk3 (CDK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Promoter

Available since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
pTBS 5Y YIC

Plasmid

#18786

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

TRAP binding site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-Tmprss6(S762A)

Plasmid

#18791

Depositor

Bruce Beutler

Article

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed Serine762 to Alanine

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pmCherry-AHPH

Plasmid

#187278

Purpose

Express mCherry-AHPH (RhoA biosensor)

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin and Pleckstrin homology domain (ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AHPH

Promoter

CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry in pmCherryN1

Plasmid

#187283

Purpose

Express IL2-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit (IL2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTripZ-Tet-ON mCherry

Plasmid

#187262

Purpose

Doxycyclin inducible expression of mCherry

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

mCherry-NLS

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-WT-GFP

Plasmid

#187258

Purpose

Lentiviral expression of myosin regulatory light chain-WT

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCavin1KDR-EGFP

Plasmid

#187254

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6, 5'LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Use

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Use

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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