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pXY029 (mEos2-MTSBs)

Plasmid

#72652

Purpose

Inducible expression of mEos2-MTSBs in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

mEos2-MTSBs

Use

Tags

mEos2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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AALN-BE4max

Plasmid

#138161

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(AALN)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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YE2-BE4max

Plasmid

#138156

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE2)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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EE-BE4max

Plasmid

#138158

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(EE)-nSpCas9-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 R126E + R132E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNG1256

Plasmid

#149710

Purpose

Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

rpoZ (rpoZ )

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-CP1028

Plasmid

#138160

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-T5-ASG_ClpXP

Plasmid

#153299

Purpose

Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

ASG

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLyGo-Bs-1

Plasmid

#163137

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Bs-2

Plasmid

#163140

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-NG

Plasmid

#138159

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-G9QLF2

Plasmid

#103111

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

G9QLF2(Cas9 coding gene from Bacillus smithii 7_3_47FAA)

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLH01

Plasmid

#117261

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-Lp_0640-42, sapK-sappR

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLH02

Plasmid

#117262

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-LVISKB_1732-30 , sapK-sappR

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pG1AK-PheB

Plasmid

#71738

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

PheB

Use

Synthetic Biology; Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Use

Tags

Expression

Bacterial

Mutation

Promoter

sppA

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Todd Klaenhammer, Rodolphe Barrangou

Article

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Use

Tags

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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