We narrowed to 1,270 results for: grna cloning vector

1. 8xCTS1_BpiI-cloning-site_SV40polyA

   Plasmid

   #124547

   Purpose

   Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT040

   Plasmid

   #67640

   Purpose

   URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Insert

   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Expression

   Yeast

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. DR274

   Plasmid

   #42250

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR; Zebrafish expression

   Expression

   Worm

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1114H

   Plasmid

   #200640

   Purpose

   An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

   Depositor

   Omar Akbari

   Article

   Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

   Insert

   gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB6627

   Plasmid

   #106159

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAJAK-71 (pESC-NatMXsyn-USER)

   Plasmid

   #78232

   Purpose

   Empty vector, for the insertion of gRNA expression cassettes into the vector.

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-UniSAM

   Plasmid

   #99866

   Purpose

   Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

   Depositor

   Lesley Forrester

   Article

   Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

   Insert

   UniSAM-mCherry + U6-gRNA2.0
   

   Use

   CRISPR and Synthetic Biology; Dcas9-sam activation

   Expression

   Mammalian

   Mutation

   BbsI sites in CDS were ablated by consensus mutat…

   Promoter

   EF1a

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJin-264

   Plasmid

   #125717

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
   

   Expression

   Mammalian

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJin-239

    Plasmid

    #125716

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
    

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFD7

    Plasmid

    #73916

    Purpose

    gRNA vector for AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    tRNA:hAsCpf1-gRNA:tRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196079

    Purpose

    Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196081

    Purpose

    Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196083

    Purpose

    Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196075

    Purpose

    Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196077

    Purpose

    Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV P2Gm +SwaI

    Plasmid

    #22699

    Depositor

    Richard Hynes

    Insert

    miR30
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pROS17

    Plasmid

    #107931

    Purpose

    TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pROS11

    Plasmid

    #107925

    Purpose

    amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge