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RBM14_pLX307

Plasmid

#98367

Purpose

Lentiviral expression of RBM14

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RBM14 (RBM14 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 prodomain-VC155

Plasmid

#49261

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBIFC-flag-hCaspase 2 prodomain-VN173

Plasmid

#49262

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Use

Tags

flag and split Venus N

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-HA-hCaspase 2 CARD-VC155

Plasmid

#49259

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

Caspase-2 CARD (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

deleted aa 123-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 CARD-VN173

Plasmid

#49260

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 CARD (CASP2 Human)

Use

Tags

Flag and split Venus N

Expression

Mammalian

Mutation

delete aa 123-435

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 FL (C303S)-VN173

Plasmid

#49258

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Use

Tags

flag and split Venus N

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pENT-L1-Kozak-GAP43-ECFP-stop-L2

Plasmid

#186356

Purpose

Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Growth-associated protein-43 (Gap43 Synthetic, Mouse)

Use

Expression of a fluorescent membrane marker

Tags

ECFP

Expression

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-KATNA1

Plasmid

#180650

Purpose

Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GGACAGCACUCCCUUGAAA

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Frt-hspB8

Plasmid

#63099

Purpose

Expression of HSPB8 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb8 (HSPB8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB2

Plasmid

#63103

Purpose

Expression of HSPB2 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb2 (HSPB2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Frt-hspB2

Plasmid

#63093

Purpose

Expression of HSPB2 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb2 (HSPB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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TPD52L3 gRNA (BRDN0001145193)

Plasmid

#76952

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPD52L3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TPD52L3 (TPD52L3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat DJ-1 shRNA

Plasmid

#222870

Purpose

Expresses mTagBFP2 along with an shRNA against rat DJ-1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 shRNA (Park7 Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-CHMP2A-myc, L216D/L219D

Plasmid

#180645

Purpose

Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP2A (CHMP2A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

L216D, L219D

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNA1 MIT residues 1-79, V55D

Plasmid

#180615

Purpose

Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-79, V55D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 R14A

Plasmid

#160052

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

R14A mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-OSF-KATNA1 V55D

Plasmid

#160053

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

V55D mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TPD52L3 gRNA (BRDN0001147599)

Plasmid

#76953

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPD52L3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TPD52L3 (TPD52L3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TPD52L3 gRNA (BRDN0001144944)

Plasmid

#76954

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPD52L3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TPD52L3 (TPD52L3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL hygro Flag HA TRAP220 wt

Plasmid

#17433

Purpose

Retroviral expression of Med1/TRAP220 in mammalian cells

Depositor

Kai Ge

Article

Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Insert

TRAP220 (MED1 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge