We narrowed to 22,191 results for: his

1. pCellFree_G06_ZIKV NS2A

   Plasmid

   #145353

   Purpose

   Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS2A
   

   Use

   Tags

   mCherry and His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b/GgVcl V1-851 (aka Vh)

   Plasmid

   #46172

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

   Insert

   Vcl 1-851 (VCL Chicken)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-851 (aka Vh) head domain

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFasBac+GFP-CAMSAP2 CC+CKK

   Plasmid

   #59039

   Purpose

   for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 CC+CKK domains (CAMSAP2 Human)
   

   Use

   Tags

   His and eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET11a-DAAT-V33G/T242G

   Plasmid

   #159278

   Purpose

   Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

   Depositor

   Roberto Chica

   Article

   Parmeggiani et al ACS Catalysis 9, 3482–3486.

   Insert

   Engineered d-alanine aminotransferase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Valine 33 and threonine 242 have been mutated to …

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G06_ZIKV PrM

   Plasmid

   #145350

   Purpose

   Cell-free expression vector for Zika virus prM protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV PrM
   

   Use

   Tags

   mCherry and His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEU3-NII-HLICNot

   Plasmid

   #140181

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   pEU3-NII-HLICNot

   Expression

   Mutation

   Promoter

   T7

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFAST-BAC-Cdc20wt (2193)

   Plasmid

   #39850

   Depositor

   Jonathon Pines

   Article

   Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

   Insert

   Cdc20 (CDC20 Human)
   

   Use

   Baculovirus expression

   Tags

   His

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQStrep2-TRAPPC3

   Plasmid

   #34711

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   trafficking protein particle complex 3 (TRAPPC3 Human)
   

   Use

   Tags

   His and StrepII-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-MRPS2

    Plasmid

    #31342

    Depositor

    Linda Spremulli

    Article

    Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

    Insert

    mitochondrial ribosomal protein S2 mature form (MRPS2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Encodes the predicted mature form for incorporati…

    Promoter

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET15b-At 14-3-3 mu

    Plasmid

    #44938

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 mu (GRF9 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pANY3

    Plasmid

    #112197

    Purpose

    This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQTEV-PHYHIP

    Plasmid

    #31578

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    phytanoyl-CoA hydroxylase interacting protein (PHYHIP Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ414 - DANCER-3

    Plasmid

    #136387

    Purpose

    Expresses DANCER-3 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-3
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-Y-GECO2m

    Plasmid

    #105070

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Use

    Tags

    6x His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BacPAK GST-Uch37N

    Plasmid

    #19421

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 1-239 (UCHL5 Human)
    

    Use

    Tags

    GST and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

17. LacD_WT_pET21a

    Plasmid

    #29785

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. LacD_L165E_pET21a

    Plasmid

    #29786

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

19. LacD_L275S_pET21a

    Plasmid

    #29787

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L275S

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. LacD_L165E/L275S_pET21a

    Plasmid

    #29788

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E and L275S

    Promoter

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pCellFree_G03 CTNNB1

    Plasmid

    #67119

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pQTEV-PHYHIPL

    Plasmid

    #34823

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    phytanoyl-CoA hydroxylase interacting protein-like (PHYHIPL Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. A102 ΔAmpH Δ2x+ve

    Plasmid

    #129484

    Purpose

    Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. A102-GAP-BoCCS-GRM

    Plasmid

    #123286

    Purpose

    Expression of Glo3 ΔAmpH (1-459) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCellFree_G03 TCF3

    Plasmid

    #67110

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Truncated isoform

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pPROEX Hsp104 (773-908)

    Plasmid

    #1316

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (773-908) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    deletion of aa1-773

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPROEX Hsp104 (361-548)

    Plasmid

    #1317

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (361-548) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa361-548 of Hsp104

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTorPE-Y-GECO2.2s

    Plasmid

    #105069

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2.2s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQTEV-MGC20398

    Plasmid

    #34825

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    MGC20398 (ZNF830 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EKAR2G_design1_mTFP_wt_Venus_173

    Plasmid

    #39815

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BacPAK HF-Uch37

    Plasmid

    #19425

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 (UCHL5 Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

33. EKAR2G_design1_mTFP_105_Venus_229

    Plasmid

    #39822

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pK RagA-RagC(Q120L)

    Plasmid

    #99701

    Purpose

    Bacterial expression of RagA-RagC(Q120L)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    Q120L

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMT mir-1-1 reporter cel-mir-40

    Plasmid

    #46739

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    cel-mir-40
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PGRP-LCa

    Plasmid

    #21059

    Depositor

    Johann Deisenhofer

    Article

    Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    PGRP-LCa (PGRP-LF Fly)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pTriEX Tom20 mVenus BoPL

    Plasmid

    #114467

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoPL
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTriEx TOM20 mVenus BoLL

    Plasmid

    #114465

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoLL

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoLL
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PGRP-LCx

    Plasmid

    #21058

    Depositor

    Johann Deisenhofer

    Article

    Chang et al Science. 2006 Mar 24. 311(5768):1761-4.

    Insert

    PGRP-LCx (PGRP-LC Fly)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

40. BacPAK GST-Uch37

    Plasmid

    #19710

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 (UCHL5 Human)
    

    Use

    Tags

    GST and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only