We narrowed to 5,563 results for: org

1. PB-CT3G-CERP2

   Plasmid

   #175499

   Purpose

   All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-ST1n-VP64

   Plasmid

   #48675

   Purpose

   Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null (D9A D598A H599A N622A)

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET23a-HsRPS27a

   Plasmid

   #69561

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pSN007

   Plasmid

   #102440

   Purpose

   For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

   Depositor

   George Church, Alejandro Chavez

   Article

   Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

   Insert

   7SK paired gRNA insert
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA_tdtomato_reporter1_SP-2x

   Plasmid

   #79370

   Purpose

   Assays activity of transcriptional activators fused to SP Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_TRE_Z17-PDGFR_EF1a_mCherry

   Plasmid

   #186303

   Purpose

   Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z17-PDGFR
   mCherry
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

   Plasmid

   #79373

   Purpose

   Recruits VPR to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hNoxo1

   Plasmid

   #58530

   Purpose

   expresses human Noxo1 in mammalian cells

   Depositor

   Karl-Heinz Krause, Botond Banfi

   Article

   Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

   Insert

   NADPH oxidase organizer 1 (NOXO1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC31

   Plasmid

   #209996

   Purpose

   Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C2SN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC32

    Plasmid

    #209997

    Purpose

    Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    3' Connector (3C5CSN)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC52

    Plasmid

    #209991

    Purpose

    Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C2SN)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC51

    Plasmid

    #209990

    Purpose

    Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C1CSN)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SMVP-Cas9N-U6-gRNA P2

    Plasmid

    #80944

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK010

    Plasmid

    #69874

    Purpose

    expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

    Depositor

    Georg Nagel, Robert Kittel

    Article

    Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

    Insert

    Channelrhodopsin-2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D156C (XXL)

    Promoter

    Drosophila Hsp70

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSV-p65T305A

    Plasmid

    #106454

    Purpose

    NF-kB p65, kinase site 305 mutated

    Depositor

    Georg Weber

    Article

    He et al Eur J Biochem. 2003 May . 270(10):2174-8

    Insert

    NF-kB p65, kinase site 305 mutated (RELA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    kinase site inactivated

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERP2-MEIOC

    Plasmid

    #222562

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    MEIOC (C17orf104 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMP-YY1_1

    Plasmid

    #36357

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pLP-mScarlet-I

    Plasmid

    #209993

    Purpose

    Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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