We narrowed to 5,692 results for: org

1. PB_TRE_sg30-PDGFR_EF1a_mCherry

   Plasmid

   #186309

   Purpose

   Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg30-PDGFR
   mCherry
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_TRE_sg61-PDGFR_EF1a_eGFP

   Plasmid

   #186310

   Purpose

   Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg61-PDGFR
   eGFP
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTurquoise2-Giantin

   Plasmid

   #129630

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   mTurquoise2-Giantin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_TRE_Z18-PDGFR_EF1a_eGFP

   Plasmid

   #186304

   Purpose

   Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z18-PDGFR
   eGFP
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_TRE_Z17-PDGFR_EF1a_mCherry

   Plasmid

   #186303

   Purpose

   Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z17-PDGFR
   mCherry
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_TRE_AP4-PDGFR_EF1a_eBFP2

   Plasmid

   #186306

   Purpose

   Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP4-PDGFR
   eBFP2
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mScarletI_MapTauC

   Plasmid

   #112964

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   MapTauC
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_TRE_sg83-PDGFR_EF1a_iRFP670

   Plasmid

   #186311

   Purpose

   Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg83-PDGFR
   iRFP670
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_TRE_sg88-PDGFR_EF1a_eBFP2

    Plasmid

    #186312

    Purpose

    Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg88-PDGFR
    eBFP2
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_TRE_P9-PDGFR_EF1a_mPlum

    Plasmid

    #186307

    Purpose

    Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P9-PDGFR
    mPlum
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB_TRE_AP10-PDGFR_EF1a_mOrange

    Plasmid

    #186308

    Purpose

    Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    AP10-PDGFR
    mOrange
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid

    #186305

    Purpose

    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P3-PDGFR
    iRFP670
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lamp1-mTurquoise2

    Plasmid

    #98828

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. hCas9_D10A

    Plasmid

    #41816

    Purpose

    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9_D10A
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A nickase

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EB3-mNeonGreen

    Plasmid

    #98881

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mScarlet-I-LaminB

    Plasmid

    #98831

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    CytERM
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORTMAGE-Pa1

    Plasmid

    #138475

    Purpose

    Enables high-efficiency recombineering in P. aeruginosa

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E36K
    

    Expression

    Bacterial

    Mutation

    E36K

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mScarlet-I-UtrCH

    Plasmid

    #98823

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    UtrCH
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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