We narrowed to 11,051 results for: HA
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Plasmid#180618PurposeBacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.DepositorInsertKATNAL1 (KATNAL1 Human)
TagsHIS-SUMOExpressionBacterialMutationResidues 1-87PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-THTAP NRBF2 MIT residues 1-111
Plasmid#180621PurposeBacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.2
Plasmid#201404PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet-Seq1.1
Plasmid#201401PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/Col1a1/GFP4_Seq 1.1
Plasmid#201400PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10-FERM-ITGBD
Plasmid#194855PurposeExpress EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.DepositorInsertMYO10 S2001_F2002insA/T2009D (MYO10 Human)
TagsEGFPExpressionMammalianMutationMYO10 has the following mutations S2001_F2002insA…PromoterCMVAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-δ
Plasmid#197259PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-3 δ (AP3D1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationVal 549 Ile substitution; silent substitutions in…PromoterADH1Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-γ1
Plasmid#197078PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-1 γ1 (Ap1g1 Mouse)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ε
Plasmid#197261PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-4 ε (AP4E1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitutions in codons 905, 1129 and 1137PromoterADH1Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Dcst1-3xHA
Plasmid#183540PurposeExpress Dcst1-3xHA under the mouse Clgn promoter.DepositorAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)
Plasmid#185314PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertpvLEA22mer_shuffle_3
ExpressionMammalianAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_D125I_mCh-2xFKBP (pBS1157)
Plasmid#185330PurposeFor the mammalian expression of the human protein ApoE3_D125I attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_D125I
ExpressionMammalianMutationD125IAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_mCh-2xFKBP (pBS1156)
Plasmid#185329PurposeFor the mammalian expression of the human protein ApoE3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136)
Plasmid#185320PurposeFor the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS1_PARRC_101-189
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYUA_HUMAN_mCh-2xFKBP (pBS1135)
Plasmid#185319PurposeFor the mammalian expression of the human protein SYUA_HUMAN attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertSYUA_HUMAN
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)
Plasmid#185318PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertpvLEA22mer_mutant_3
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)
Plasmid#185317PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertPvLEA4_repeats_k5_1
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)
Plasmid#185316PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertpvLEA22mer_mutant_10
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAHS2_PARRC_98-130_mCh-2xFKBP (pBS1120)
Plasmid#185315PurposeFor the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS2_PARRC_98-130
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)
Plasmid#185313PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertPvLEA4_repeats_k3_26
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHN_VrDHN1a_mCh-2xFKBP (pBS1117)
Plasmid#185312PurposeFor the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertDHN_VrDHN1a
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116)
Plasmid#185311PurposeFor the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertDSUP_RAMVA_1-208
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHR93_Nccapped_mCh-2xFKBP (pBS1115)
Plasmid#185310PurposeFor the mammalian expression of the synthetic protein DHR93_Nccapped attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertDHR93_Nccapped
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)
Plasmid#185309PurposeFor the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_D125I_47-300
ExpressionMammalianMutationD125IAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_47-300_mCh-2xFKBP (pBS1113)
Plasmid#185308PurposeFor the mammalian expression of the human protein ApoE3_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_47-300
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE4_47-300_mCh-2xFKBP (pBS1112)
Plasmid#185307PurposeFor the mammalian expression of the human protein ApoE4_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE4_47-300
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE2_47-300_mCh-2xFKBP (pBS1111)
Plasmid#185306PurposeFor the mammalian expression of the human protein ApoE2_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE2_47-300
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAHS3hypdu-mCh-2xFKBP (pBS1110)
Plasmid#185305PurposeFor the mammalian expression of the tardigrade protein CAHS3hypdu attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS3hypdu
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Dcst2-3xHA
Plasmid#183541PurposeExpress Dcst2-3xHA under the mouse Clgn promoter.DepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC002
Plasmid#171139PurposeFor integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertsJ1-BBa_J23117-sfGFP
Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterJ1-BBa_J23117 and Sp.pCas9, BBa_J23107Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK R106A p2A mCherry
Plasmid#117688PurposeCMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencingDepositorInsertBARKrgs R106A peptide
TagsHA / FLAGExpressionMammalianMutationR106AAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1A Prk-
Plasmid#162694Purposep1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialMutationPrk inactive (K20M S21A native protein, this map …PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_RKK/AAA POLI
Plasmid#131240PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530ADepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains point mutations that convert arginine re…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S433A POLI
Plasmid#131235PurposeMammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S497A POLI
Plasmid#131236PurposeMammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S520A POLI
Plasmid#131237PurposeMammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S580A POLI
Plasmid#131238PurposeMammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only