We narrowed to 18,127 results for: Mut

1. pDC10

   Plasmid

   #75077

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Insert

   phosphoribosylglycinamide synthetase (purD Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   adds unique SacII site at Thr95-Ala96 by a silent…

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-HseIF4AI_T

   Plasmid

   #147543

   Purpose

   Insect Expression of HseIF4AI

   Depositor

   Elisa Izaurralde

   Insert

   HseIF4AI (EIF4A1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   3 silent mutations compared to the sequence given…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pb-CAGGS-dCas9-blasto

   Plasmid

   #218555

   Purpose

   Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance

   Depositor

   Alejandro Chavez

   Insert

   dCas9
   

   Use

   CRISPR; Piggybac transposition

   Tags

   2xSV40NLS

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

   Plasmid

   #100050

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV9

   Insert

   hChR2(E123T/H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   humanized, E123T/H134R

   Promoter

   CaMKIIa

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmCer-Mcm5

   Plasmid

   #211391

   Purpose

   mammalian expression of human Mcm5 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Mcm5 (MCM5 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEGFP

   Plasmid

   #18696

   Depositor

   Karel Svoboda

   Article

   Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

   Insert

   monomeric EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K mutation

   Promoter

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-hChR2(H134R)-mCherry

   Plasmid

   #26976

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV5, AAV8, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-GFAP-hM3D(Gq)-mCherry

   Plasmid

   #50478

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-GFAP-hM4D(Gi)-mCherry

   Plasmid

   #50479

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19_GINS4_dTAG

    Plasmid

    #232735

    Purpose

    Generates GINS4 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    GINS4 (GINS4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIND-HA-ADH

    Plasmid

    #11991

    Depositor

    Steven Johnson

    Insert

    aldehyde dehydrogenase (ALDH1A1 Human)
    

    Use

    Ecdysone inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pmVen-DHX9

    Plasmid

    #211409

    Purpose

    mammalian expression of human DHX9 N-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b-12x

    Plasmid

    #61699

    Purpose

    Expresses PTDH mutant Opt12x

    Depositor

    Huimin Zhao

    Article

    Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

    Insert

    Opt12x
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. paGFP

    Plasmid

    #18697

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    photoactivatable GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    CMV

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-IGFBP5-V5

    Plasmid

    #11608

    Depositor

    Steven Johnson

    Insert

    Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pZA31-sulA-GFP

    Plasmid

    #78493

    Purpose

    Expression of GFP under the sulA promoter.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCer-DHX9

    Plasmid

    #211408

    Purpose

    mammalian expression of human DHX9 N-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. poRibo-T2

    Plasmid

    #69347

    Purpose

    Ribo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutation

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    oRibo-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-V5-SBP-C1-HsUpf1_1-925-siRNAres_P

    Plasmid

    #147191

    Purpose

    Mammalian Expression of HsUpf1_1-925-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    HsUpf1_1-925-siRNAres (UPF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    4 non silent mutations A69S, E167G, K282R and A4…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT7-V5-SBP-C1-HsUpf1-del296-926_P

    Plasmid

    #147195

    Purpose

    Mammalian Expression of HsUpf1-del296-926

    Depositor

    Elisa Izaurralde

    Insert

    HsUpf1-del296-926 (UPF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    4 non silent mutations A69S, E167G, K282R and T1…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT7-V5-SBP-C1-HsUpf1_115-1129-siRNAres_P

    Plasmid

    #147193

    Purpose

    Mammalian Expression of HsUpf1_115-1129-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    HsUpf1_115-1129-siRNAres (UPF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 non silent mutations A69S, E167G, K282R, A442G…

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-V5-SBP-C1-HsUpf1-V161ER162E-siRNAres_Q

    Plasmid

    #147332

    Purpose

    Mammalian Expression of HsUpf1-V161ER162E

    Depositor

    Elisa Izaurralde

    Insert

    HsUpf1-V161ER162E (UPF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 non silent mutations A69S, E167G, K282R, A442G…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG1guide_RUBY_EggCas9

    Plasmid

    #225983

    Purpose

    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

    Depositor

    Imran Siddiqi

    Insert

    Guide RNA against AtAGAMOUS1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35s promoter to Drive RUBY and DD45p to drive Cas9

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-HA-CIRBP

    Plasmid

    #11607

    Depositor

    Steven Johnson

    Insert

    Cold Inducible RNA Binding Protein (CIRBP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pGFP FAK Y397F

    Plasmid

    #50516

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y397F

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pAd/WT1-IRES-nAmCyan

    Plasmid

    #29756

    Depositor

    Edward McCabe

    Insert

    Wilms tumor 1 (WT1 Human)
    

    Use

    Tags

    AmCyan, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; AmCyan is nuclear localized; WT1 an…

    Promoter

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT7-MS2-HA-C1-HsYTHDF1_AB

    Plasmid

    #148316

    Purpose

    Mammalian Expression of HsYTHDF1

    Depositor

    Elisa Izaurralde

    Insert

    HsYTHDF1 (YTHDF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFPV25

    Plasmid

    #20667

    Depositor

    Raphael Valdivia

    Article

    Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

    Type

    Empty backbone

    Use

    Tags

    GFP mut3

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-FlagmuEzh2deltaset-Hygro

    Plasmid

    #49403

    Purpose

    Expresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.

    Depositor

    Tobias Neff

    Insert

    3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Set domain deleted

    Promoter

    MSCV promotor

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

31. GBH-R-cmlc2GFP.1

    Plasmid

    #188004

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker cmlc2 GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; cmlc2 promotor

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GBH-R-gcryGFP.3

    Plasmid

    #188000

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAd/CMV/PBX1-IRES-nEBFP2

    Plasmid

    #29755

    Depositor

    Edward McCabe

    Insert

    Pre-B-cell leukemia transcription factor 1 (PBX1 Human)
    

    Use

    Tags

    EBFP2, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; EBFP2 is nuclear localized; PBX1 an…

    Promoter

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

34. pCAGGS-Flag-S1pr1-R120A

    Plasmid

    #203707

    Purpose

    Overexpression of FLAG-S1PR1-R120A mutant.

    Depositor

    Timothy Hla

    Insert

    Flag-S1PR1-R120A (S1PR1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R120A

    Promoter

    Available Since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSET-QUEEN 7mu A81D

    Plasmid

    #129351

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu A81D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSET-QUEEN 7mu E83A

    Plasmid

    #129353

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu E83A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTY1048-S65T-LacI

    Plasmid

    #34853

    Depositor

    Paul Sternberg

    Article

    Gonzalez-Serricchio et al BMC Genet. 2006 Jun 7;7:36.

    Insert

    GFP-LacI
    

    Use

    Tags

    Expression

    Worm

    Mutation

    GFP is mutated: S65T and contains introns, in ord…

    Promoter

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid

    #100049

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    hChR2(E123T/H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized, E123T/H134R

    Promoter

    hSynap

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmBicaudalC_P

    Plasmid

    #147163

    Purpose

    Insect Expression of DmBicaudalC

    Depositor

    Elisa Izaurralde

    Insert

    DmBicaudalC (BicC Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    12 silent and three non silent mutattions I331T, …

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MUM1

    Plasmid

    #28273

    Depositor

    Cheryl Arrowsmith

    Insert

    MUM1 (MUM1 Human)
    

    Use

    Tags

    His with TEV cleavage

    Expression

    Bacterial

    Mutation

    Contains PWWP Domain (residues 405-538).

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits