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pX458_sgRNA_Ago1_6

Plasmid

#172471

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1.

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ gcsf

Plasmid

#132947

Purpose

Expresses zebrafish granulocyte colony stimulating factor (csf3a)

Depositor

Jason Berman

Article

Rajan et al Leukemia. 2021 Sep 30. pii: 10.1038/s413

Insert

gcsf (csf3a Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E18: attB4-polyhedrinp>-attB5

Plasmid

#162977

Purpose

Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pZS160

Plasmid

#134238

Purpose

Lentiviral plasmid expressing sgRNA against HJURP

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human HJURP sgRNA

Use

Lentiviral

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pKM328

Plasmid

#134243

Purpose

Mammalian expression construct expressing sgRNA against PLK1

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

gRNA targeting PLK1 3'

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-TFDP2

Plasmid

#114300

Purpose

Constitutive expression of the TFDP2 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP2 (TFDP2 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLV(shRNA)-Puro-6 CCEPR Vb2

Plasmid

#108278

Purpose

shRNA for CCEPR lncRNA

Depositor

Karl Munger

Article

Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

Insert

CCEPR (CCEPR Human)

Use

Lentiviral

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 R138S

Plasmid

#87659

Purpose

Flag/HA tagged HPV8 E6 R138S

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

R138S

Promoter

CMV

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K64A

Plasmid

#87656

Purpose

Flag/HA tagged HPV8 E6 K64A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

K64A

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K136N

Plasmid

#87657

Purpose

Flag/HA tagged HPV8 E6 K136N

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

K136N

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K142A

Plasmid

#87660

Purpose

Flag/HA tagged HPV8 E6 K142A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

K142A

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 L59D

Plasmid

#87655

Purpose

Flag/HA tagged HPV8 E6 L59D

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

L59D

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV MmuPV1 E6 R130A

Plasmid

#87654

Purpose

Flag/HA tagged MmuPV1 E6 R130A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MmuPV1 E6

Tags

Flag and HA

Expression

Mammalian

Mutation

R130A

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBW215J105-Amp32C

Plasmid

#78650

Purpose

pSB3K3 carrying J105-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J105-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW212J114-Amp32C

Plasmid

#78647

Purpose

pSB3K3 carrying J114-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J114-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pIhh4.5k-Luc

Plasmid

#53603

Purpose

Reporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

Ihh promoter

Use

Luciferase; Reporter

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut2

Plasmid

#51850

Purpose

promoter region of PIM2 lacking the forkhead binding site #1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
roGFP_iR_pQE30

Plasmid

#48634

Purpose

Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

roGFP1

Tags

HisTag

Expression

Bacterial

Mutation

insertion R147B

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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