We narrowed to 6,674 results for: SIM;

1. pcDNA5-FRT-TO-His6-NUbo-H2B-His8

   Plasmid

   #212811

   Purpose

   Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   H2B
   

   Use

   Tags

   6xHis-NUbo and 8xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT-TO-His8-H2B-CUbo

   Plasmid

   #212812

   Purpose

   Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   H2B
   

   Use

   Tags

   8xHis and CUbo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

   Plasmid

   #212813

   Purpose

   Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   H2B
   

   Use

   Tags

   8xHis and CUbo(K48R)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMZ828

   Plasmid

   #155322

   Purpose

   Constitutive expression of PhyB(1-650)-VP16 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pROF366

   Plasmid

   #155332

   Purpose

   Expression of FLuc under the control of AtAP1 promoter

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PAtAP1-FLuc-TSV40
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

   Plasmid

   #199447

   Purpose

   Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   dSaCas9-10xGCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICH47811-pAtAct2::Cas9-nls::tSlATPase

   Plasmid

   #160221

   Purpose

   Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtACT2::Cas9-nls::tSlATPase
   

   Use

   Synthetic Biology; Moclo toolkit level 1 acceptor…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEM-T Easy-Cas9-nls-CDS1

   Plasmid

   #160231

   Purpose

   Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
   

   Use

   Level 0 of moclo golden gate position cds1

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2-npas4l

   Plasmid

   #164654

   Purpose

   for the synthesis of zebrafish npas4l mRNA

   Depositor

   Didier Stainier

   Article

   Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

   Insert

   npas4l (npas4l Zebrafish)
   

   Use

   Mrna synthesis

   Tags

   Expression

   Mutation

   Please see depositor comments

   Promoter

   SP6

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2z-etsrp

    Plasmid

    #164655

    Purpose

    for the synthesis of zebrafish etsrp mRNA

    Depositor

    Didier Stainier

    Article

    Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

    Insert

    etsrp (etsrp Zebrafish)
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM33_CRK_SH3

    Plasmid

    #178472

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CRK_SH3 (CRK Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM33_IRF3_SMAD/FHA

    Plasmid

    #178479

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    IRF3_IRF3 (IRF3 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ414-PCY1:S495A

    Plasmid

    #98151

    Purpose

    Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:S495A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed serine 495 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJ414-PCY1:W603A

    Plasmid

    #98153

    Purpose

    Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:W603A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tryptophan 603 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJ414-PCY1:H695Q

    Plasmid

    #98154

    Purpose

    Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695Q
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to glutamine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ414-PCY1:H695A

    Plasmid

    #98155

    Purpose

    Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmEDC3_441-680_D

    Plasmid

    #146127

    Purpose

    Insect Expression of DmEDC3_441-680

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3_441-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    3 mutations N162T, K221E and D418G compared to th…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmEDC3_441-680_D

    Plasmid

    #146130

    Purpose

    Insect Expression of DmEDC3_441-680

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3_441-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge