We narrowed to 19,151 results for: Tat

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pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

Plasmid

#174029

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-mScarlet

Use

Zebrafish knock-in tagging

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PLXNB2

Plasmid

#86236

Purpose

PLXNB2 full-length human cDNA CDS in gateway entry plasmid

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

PLXNB2 (PLXNB2 Human)

Use

Gateway Cloning

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK1

Plasmid

#138692

Purpose

Expresses a human NUAK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK1 human (NUAK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.ELREA746del_T790M

Plasmid

#82917

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

T790M; p.ELREA746del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-hdNadV

Plasmid

#83362

Purpose

Expresses nadV from Haemophilus ducreyi in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

Insert

nadV

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-intron-BaLgp160

Plasmid

#100918

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env with upstream intron

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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LUC45 (L/Sec/mutP)

Plasmid

#112148

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)

Expression

Mammalian

Mutation

Cysteine 258 mutated to selenocysteine (U) and SE…

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shMLL-AF9.1039

Plasmid

#105566

Purpose

retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

shRNA targeting MLL part of MLL-AF9 fusion

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
pmCherry-Synaptojanin-1-145

Plasmid

#223563

Purpose

Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

SYNJ1 (SYNJ1 Human)

Tags

His and mCherry

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(ER-mito)

Plasmid

#214427

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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p006-GFP-weak

Plasmid

#108314

Purpose

GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

pFAB4282

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337V

Plasmid

#98674

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

M337V

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4/Cre

Plasmid

#89651

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-LYY-YFP

Plasmid

#75271

Purpose

G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

G-protein independent version of optoB2

Tags

eYFP

Expression

Mammalian

Mutation

point mutations at L72F,Y136G,Y224A

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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shGLDC-DOX

Plasmid

#72883

Purpose

dox inducible knockdown

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

RNAi hairpin against GLDC

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
pUAST cyto Grx1-roGFP2

Plasmid

#64994

Purpose

for expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
NTSP-PC1

Plasmid

#41575

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

Myc/Myc/His

Expression

Mammalian

Mutation

4106-4218 of FLS PC1

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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