We narrowed to 9,265 results for: yeast expression

1. pMOD8A-iRGR-r11

   Plasmid

   #74369

   Purpose

   This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r11
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AHD1

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSN074

   Plasmid

   #166731

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EZ-L767

   Plasmid

   #131773

   Purpose

   PADH1_sFUS_Fusion Red_PixD_TACT1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_sFUS_Fusion Red_PixD_TACT1
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PP363

   Plasmid

   #78984

   Purpose

   255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Inserts

   rHGH
   IFNa2b
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAH1

   Plasmid

   #41825

   Purpose

   Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7, TEF

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EZ-L498

   Plasmid

   #131771

   Purpose

   PPGK1_FUSN_Citrine _PixE_TCYC1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PPGK1_FUSN_Citrine _PixE_TCYC1
   

   Use

   Integration into his3 locus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSN069

   Plasmid

   #166728

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-Mxi1
   

   Use

   CRISPR

   Tags

   Mxi1

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS316-RGR-GFP-mHDV

   Plasmid

   #51058

   Purpose

   Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS316-RGR-GFP-mHH

    Plasmid

    #51057

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA3

    Plasmid

    #46790

    Purpose

    To express ATPase inactive Fun30 in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    ATP-binding site mutated replaced lysin 603 by ar…

    Promoter

    PGAL1

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pDR-AmTrac

    Plasmid

    #47770

    Purpose

    expresses AmTrac in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PP165

    Plasmid

    #78985

    Purpose

    Supplemental Figure 4. Promoter GCW14. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PP275

    Plasmid

    #78968

    Purpose

    Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS406 ATG9-GFP S122D

    Plasmid

    #114667

    Purpose

    Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    changed serine 122 to aspartic acid

    Promoter

    ATG9

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDR-AmTrac1;2

    Plasmid

    #47765

    Purpose

    expresses AmTrac1;2 in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac1;2
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available Since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pDR-AmTrac-LS

    Plasmid

    #47767

    Purpose

    expresses AmTrac-LS in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac-LS
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

18. p413GPD-hTPI Lys13Arg

    Plasmid

    #50722

    Purpose

    expression of the human TPI Lys13Arg allele in yeast

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    GPD

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PP161

    Plasmid

    #78993

    Purpose

    Supplemental Figure 4. Promoter GAP3. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PP164

    Plasmid

    #78988

    Purpose

    Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge