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We narrowed to 10,047 results for: pET

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Showing: 4841 - 4860 of 10047 results

  1. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid
    #73215
    Purpose
    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    EGFR (EGFR Human, Schistosoma japonicum, Synthetic)
    Tags
    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73215-plasmid-map-sequence-id-137064

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  2. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)

    Plasmid
    #223784
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Publication
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Tags
    EGFP-TEV-GST
    Expression
    Bacterial
    Mutation
    I224A/L227A/W276A/I279A/I307A/V310A; deletion of …
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223784-plasmid-map-sequence-id-448415

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  5. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162673-plasmid-map-sequence-id-321521

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  6. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162686-plasmid-map-sequence-id-321468

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  7. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162687-plasmid-map-sequence-id-321457

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  9. pET28a(+)-LMNA-ThrHis-Cter

    Plasmid
    #256366
    Purpose
    Human full length lamin A (LMNA) with C-terminal 6x-His tag and cleavable thrombin side. For expression in E. coli ArcticExpress at 24 h, 11-15°C.
    Depositor
    Andrei Lupas
    Insert
    LMNA (LMNA Human)
    Tags
    His-tag: C-terminal which can be cleaved with thr…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    256366-plasmid-map-sequence-id-523675

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  10. pET28a(+)-LMNA-His-Cter

    Plasmid
    #256367
    Purpose
    Human full length lamin A (LMNA) with C-terminal 6x-His tag WITHOUT thrombin cleavage site. For expression in E. coli ArcticExpress at 24 h, 11-15°C.
    Depositor
    Andrei Lupas
    Insert
    LMNA (LMNA Human)
    Tags
    His-tag: C-terminal which CANNOT be cleaved with …
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    256367-plasmid-map-sequence-id-523676

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  13. pET28-MHL_IFIH1:306-1017

    Plasmid
    #247536
    Purpose
    E. coli expression for protein production. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    IFIH1 (IFIH1 Human)
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    VAR_021594 (H843R) and VAR_021595 (A946T) from te…
    Promoter
    T7
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    247536-plasmid-map-sequence-id-515932

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  14. pET28-MHL_IFIH1:306-890

    Plasmid
    #247538
    Purpose
    E. coli expression for protein production. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    IFIH1 (IFIH1 Human)
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    VAR_021594 (H843R) from template, loop deletion o…
    Promoter
    T7
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    247538-plasmid-map-sequence-id-515936

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  20. pET28a HaloTag-TEVc-6xtrTUBE

    Plasmid
    #254153
    Purpose
    Bacterial expression of 6xtrTUBE with an N-terminal HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    6xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    HaloTag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254153-plasmid-map-sequence-id-511309

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Showing: 4841 - 4860 of 10047 results