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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 14,607 results for: pET

Showing: 4841 - 4860 of 14607 results

  2. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

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  3. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T2
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97328-plasmid-map-sequence-id-182618

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  4. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid
    #140840
    Purpose
    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    Use
    Tags
    CsoS2 N-peptide consensus to CbbL C-terminus and ā€¦
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140840-plasmid-map-sequence-id-296743

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  6. pAM59

    Plasmid
    #227655
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Ī”78ā€“81)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, Ī”78ā€“81
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227655-plasmid-map-sequence-id-455288

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  11. pSLS.544

    Plasmid
    #184961
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184961-plasmid-map-sequence-id-376648

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  14. pEUK035

    Plasmid
    #180827
    Purpose
    T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    SwGdmB
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180827-plasmid-map-sequence-id-355564

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  15. pEUK036

    Plasmid
    #180828
    Purpose
    T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    CnGdmA
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180828-plasmid-map-sequence-id-355557

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  16. pEUK066

    Plasmid
    #180830
    Purpose
    T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    NaGdmA
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180830-plasmid-map-sequence-id-355559

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  18. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted theā€¦
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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Showing: 4841 - 4860 of 14607 results