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pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

3' splice site muattion

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pML2(+)BR

Plasmid

#108336

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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PdarC1

Plasmid

#24835

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PdarC1

Tags

HA and Myc

Expression

Yeast

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam EB-1

Plasmid

#16474

Depositor

Bert Vogelstein

Article

Su et al Cancer Res. 1995 Jul 15. 55(14):2972-7.

Insert

EB-1 (MAPRE1 Human)

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
NKIRAS2

Plasmid

#55676

Purpose

expression of mouse NKIRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NKIRAS2 (Nkiras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
NKIRAS1

Plasmid

#55675

Purpose

expression of mouse NKIRAS1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NKIRAS1 (Nkiras1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pCI_GFPnes

Plasmid

#240501

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Type

Empty backbone

Tags

GFPnes

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pT7RNAPNev-NB1

Plasmid

#216336

Purpose

Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

Depositor

Mark Styczynski

Article

McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

Insert

T7RNAPN-NB1 fusion

Expression

Bacterial

Available Since

July 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 JDM16-GFPnb-mCh

Plasmid

#235667

Purpose

Expresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM16-GFPnb-mCh (Mch Synthetic)

Tags

GFP nanobody and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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(P6f)3(P14f)3-gs

Plasmid

#228082

Purpose

Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

P6f, P14f

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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