We narrowed to 43,420 results for: cha;

1. Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

   Plasmid

   #26571

   Depositor

   Diane Lipscombe

   Article

   Lin et al Neuron. 1997 Jan . 18(1):153-66.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Deletion of exons a10, 18a, and 31a Contains e…

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

2. SNX6-PX (27-171)

   Plasmid

   #119087

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX6-PX (27-171)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX6-PX (29-170) (SNX6 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

   Plasmid

   #26568

   Depositor

   Diane Lipscombe

   Article

   Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   contains exons a10, 18a, 31a, 37b, and 46. Del…

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. mCherry-MyosinIIC-N-18

   Plasmid

   #55108

   Purpose

   Localization: Cytoskeleton, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

   Insert

   MyosinIIC
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSETa-mEos3.2-A69T

   Plasmid

   #98571

   Purpose

   photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos3.2-A69T
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. N-BLa 1.1

   Plasmid

   #88994

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.1 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pECE-M2-PAK2 S20A

   Plasmid

   #31681

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 S20A (PAK2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 20 to Alanine

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SH3PXD2A-PX (1-126)

   Plasmid

   #119115

   Purpose

   Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SH3PXD2A-PX (1-126) (SH3PXD2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p164 Dync1i1.B

    Plasmid

    #26435

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pEF Doc2alpha WT GFP

    Plasmid

    #128819

    Purpose

    To visualize Doc2alpha WT in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ps226

    Plasmid

    #133557

    Purpose

    RNAi construct for knock-down of CDKN1A (Cip1, p21)

    Depositor

    David Livingston

    Article

    Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTG-mTDG-K341R

    Plasmid

    #52267

    Purpose

    Bacterial expression of mouse TDG-K341R with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K341R, SUMO acceptor site mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNH12 (pmyo-2::MacQ::mCitrine)

    Plasmid

    #130274

    Purpose

    Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    MacQ::mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pScalps ACE2 hygro

    Plasmid

    #184786

    Purpose

    Stable expression of Human Ace2

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PD-L1-miSFIT-1x

    Plasmid

    #124685

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C-BLa 1.1

    Plasmid

    #88997

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    C-BLa 1.1 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-PIWI(aa478-860)

    Plasmid

    #21543

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    contain only aa478-860

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-Syn-ChromeQ-GFP

    Plasmid

    #123317

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    Syn

    Available since

    March 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTA231-p.Syn-Dendra2Hfx

    Plasmid

    #164660

    Purpose

    expression of H. volcanii codon-optimized Dendra2

    Depositor

    Anita Marchfelder

    Article

    Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

    Insert

    Dendra2Hfx
    

    Use

    Archaeal expression

    Tags

    Expression

    Mutation

    codon-optimization for Haloferax volcanii

    Promoter

    p.Syn

    Available since

    Feb. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFSW Doc2alpha WT

    Plasmid

    #128812

    Purpose

    To make virus expressing Doc2alpha WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFSW Doc2beta WT

    Plasmid

    #128814

    Purpose

    To make virus expressing Doc2beta WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV iGluf

    Plasmid

    #105833

    Purpose

    Fast indicator for extracellular glutamate imaging

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pclbw-PAGFPomp25

    Plasmid

    #58427

    Purpose

    Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    paGFP-omp25 (Synj2bp Mouse)
    

    Use

    Retroviral

    Tags

    PAGFP

    Expression

    Mammalian

    Mutation

    contains aa's 109-145 of mouse omp25

    Promoter

    cmv-bactin

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

26. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26570

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pMiR-E6AP-3'UTR

    Plasmid

    #53694

    Purpose

    Luciferase reporter assay for microRNA binding on E6AP 3'UTR

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    UBE3A 3'UTR (UBE3A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

28. p199 Dync1i2.C (Ex1a)

    Plasmid

    #26449

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

29. FLAG-PAZ(aa1-480)

    Plasmid

    #21529

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    contain only aa1-480

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

    Plasmid

    #114378

    Purpose

    expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-KA2N-EGFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EGFP and KA2N

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEF NS34a-msGFP-'Q-mito

    Plasmid

    #158764

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mEmerald-MyosinIIC-C-18

    Plasmid

    #54194

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

    Insert

    MyosinIIC
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

34. pGMC00013

    Plasmid

    #172525

    Purpose

    sgRNA against mouse Mr1

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Mr1 (Mr1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLJC6-Flag CASTOR1

    Plasmid

    #84496

    Purpose

    Lentiviral expression of CASTOR1

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    sestrin2

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

36. SNX11-PX (7-167)

    Plasmid

    #119092

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX11-PX (7-167) (SNX11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SNX12-PX (1-162)

    Plasmid

    #119093

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX12-PX (1-162)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX12-PX (1-162) (SNX12 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMpGWB321

    Plasmid

    #68649

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMpGWB119

    Plasmid

    #68573

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PD-L1-miSFIT-4x

    Plasmid

    #124684

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge