We narrowed to 19,813 results for: KIN

1. iCyt_BrEt_SnFR

   Plasmid

   #177740

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCyt_BrEt_SnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. iCyt_F_SnFR

   Plasmid

   #177739

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCyt_F_SnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mILK-R349A-EFGP

   Plasmid

   #176898

   Purpose

   mouse ILK with R349A point mutation cloned into pEGFP-N1

   Depositor

   Sara Wickström

   Article

   Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   Integrin-linked kinase (Ilk Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arginine 349 to Alanine

   Promoter

   CNV

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mILK-R255A-EFGP

   Plasmid

   #176897

   Purpose

   mouse ILK with R255A point mutation cloned into pEGFP-N1

   Depositor

   Sara Wickström

   Article

   Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   Integrin-linked kinase (Ilk Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arginine 255 to Alanine

   Promoter

   CNV

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

   Plasmid

   #167199

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R S364L
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

   Plasmid

   #167200

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R D421N
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

   Plasmid

   #167198

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E294K
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

   Plasmid

   #167197

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R M173I
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

   Plasmid

   #167196

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E144K
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQE30-VRN1

    Plasmid

    #159531

    Purpose

    Expresses Arabidopsis thaliana VERNALIZATION1 in E. coli

    Depositor

    Joshua Mylne

    Article

    King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

    Insert

    VERNALIZATION1 (VRN1 Mustard Weed)
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

    Plasmid

    #81239

    Purpose

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMinDis.X513-iNicSnFR3b-(V7)-ER

    Plasmid

    #125123

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3a-(V7)-ER
    

    Use

    Tags

    Fusion protein -Endoplasmic retention motif

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHHM.X513-iNicSnFR3a-(CC93)

    Plasmid

    #124880

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3a-(CC93)
    

    Use

    Tags

    HA Tag and His Tag

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHHM.X513-iNicSnFR2-(CC90)

    Plasmid

    #124836

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR2-(CC90)
    

    Use

    Tags

    HA tags and His Tag

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAK4-FL-3'UTR-MT2

    Plasmid

    #113093

    Purpose

    Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Second predicted miR-199a-3p binding site mutated…

    Promoter

    PGK

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PAK4-FL-3'UTR-MT-both

    Plasmid

    #113094

    Purpose

    Luciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutated

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Both the predicted miR-199a-3p binding site muta…

    Promoter

    PGK

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge