We narrowed to 9,680 results for: otos

1. pMK395 (AAVS1 CMV-OsTIR1(F74A))

   Plasmid

   #159992

   Purpose

   Expression of OsTIR1(F74A) in human cells

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Voltron

   Plasmid

   #119033

   Purpose

   Express Voltron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF-p15A-YFP

   Plasmid

   #186563

   Purpose

   p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   None

   Expression

   Bacterial and Mammalian

   Mutation

   None

   Promoter

   Promotorless

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

   Plasmid

   #113888

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

   Plasmid

   #199354

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174883

   Purpose

   Targeting vector for generating Pou5f1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Pou5f1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-N-HA-Pcdh15_mini_V4

   Plasmid

   #199185

   Purpose

   An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

   Depositor

   David Corey

   Article

   Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

   Insert

   HA-mmPcdh15_mini_V4-CD2
   

   Use

   AAV

   Tags

   Human influenza hemagglutinin (HA)

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-eYFP

   Plasmid

   #104055

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE32-pr-mEosFP (mEosFP-V69T)

   Plasmid

   #99213

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEosFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T for primed c…

   Promoter

   T5

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-GFAP-iGABASnFR2-WPRE

    Plasmid

    #218871

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    GFAP

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME Actin-Vhh sfGFP (JDW 1215)

    Plasmid

    #224495

    Purpose

    Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-sfGFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-FLEX-FLIM-AKAR

    Plasmid

    #60445

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Abl-His6-FLAG

    Plasmid

    #52684

    Purpose

    expression of Abl in mammalian cells

    Depositor

    Benjamin Turk

    Article

    Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2.

    Insert

    ABL1 (Abl1 Mouse)
    

    Use

    Tags

    FLAG and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IB-mRuby3-FTH1

    Plasmid

    #192804

    Purpose

    Stable expression of mRuby3-FTH1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6GRNA

    Plasmid

    #68370

    Purpose

    gRNA scaffold with hU6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174882

    Purpose

    Targeting vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Nanog
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-FAS-GCaMP6f-WPRE

    Plasmid

    #216277

    Purpose

    AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human synapsin 1 (hSyn)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSKC11 - UTRQ

    Plasmid

    #110490

    Purpose

    Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

    Depositor

    Shankar Balasubramanian

    Article

    Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

    Insert

    NRAS 5'-UTR (bp 1-254) (NRAS Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDule2-Acd82

    Plasmid

    #174100

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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