We narrowed to 5,233 results for: puc
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Plasmid#218283PurposeUse together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEu2
Plasmid#218282PurposeUse together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locusDepositorInsertHO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJE13HD7
Plasmid#218281PurposeUse together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.DepositorInsertHO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Luciferase-CD4-bla
Plasmid#189985PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-851_HA-GD2-28z_HA-TFAP4
Plasmid#207501PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertHA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15488_HeF-ABE8e
Plasmid#174126Purposeplasmid expressing HeF-ABE8eDepositorInsertHeF-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15487_evo-ABE8e
Plasmid#174125Purposeplasmid expressing evo-ABE8eDepositorInsertevo-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15486_HypaR661A-ABE8e
Plasmid#174124Purposeplasmid expressing HypaR661A-ABE8eDepositorInsertHypaR-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR
Plasmid#165043PurposeRepair template for CAS9 complex genome editingDepositorInsertRPL13A (RPL13A Human)
ExpressionBacterialAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvCYP76AD1
Plasmid#162529PurposeBeta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertCYP76AD1
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MjcDOPA5GT
Plasmid#162530PurposeMirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertcDOPA5GT
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVR
Plasmid#84718PurposeE. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilisDepositorInsertsE. coli RecA promoter
5' UTR of queC gene
ExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF174-donor
Plasmid#112301PurposeCRISPR donor plasmid to tag human transcription factor ZNF174 with GFPDepositorInsertZNF174 homology arms flanking EGFP-IRES-Neo cassette (ZNF174 Human)
UseCRISPRMutationrs37813, rs39729Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCF15-donor
Plasmid#113830PurposeCRISPR donor plasmid to tag human transcription factor TCF15 with GFPDepositorInsertTCF15 homology arms flanking EGFP-IRES-Neo cassette (TCF15 Human)
UseCRISPRAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHOXC13-donor
Plasmid#113827PurposeCRISPR donor plasmid to tag human transcription factor HOXC13 with GFPDepositorInsertHOXC13 homology arms flanking EGFP-IRES-Neo cassette (HOXC13 Human)
UseCRISPRAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFP3-donor
Plasmid#113811PurposeCRISPR donor plasmid to tag human transcription factor ZFP3 with GFPDepositorInsertZFP3 homology arms flanking EGFP-IRES-Neo cassette (ZFP3 Human)
UseCRISPRMutationrs11313283Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF397-donor
Plasmid#112381PurposeCRISPR donor plasmid to tag human transcription factor ZNF397 with GFPDepositorInsertZNF397 homology arms flanking EGFP-IRES-Neo cassette (ZNF397 Human)
UseCRISPRMutationrs2278463Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELF4-donor
Plasmid#112371PurposeCRISPR donor plasmid to tag human transcription factor ELF4 with GFPDepositorInsertELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
UseCRISPRAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF331-donor
Plasmid#112365PurposeCRISPR donor plasmid to tag human transcription factor ZNF331 with GFPDepositorInsertZNF331 homology arms flanking EGFP-IRES-Neo cassette (ZNF331 Human)
UseCRISPRAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only