We narrowed to 162,717 results for: addgene

1. pLL3.7 shRNA against CRTC1

   Plasmid

   #72710

   Purpose

   Lentiviral expression of shRNA against CRTC1 (3rd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   CRTC1
   

   Use

   Lentiviral

   Tags

   CMV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

2. PB-eDIO-ChR2-EYFP

   Plasmid

   #104387

   Purpose

   PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Channelrhodopsin 2
   

   Use

   Cre/Lox; Piggybac

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E134R

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CENPF C5.1 gRNA

   Plasmid

   #90616

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPF

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPF (Guide Designation C5.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CLDN17_pCSdest

   Plasmid

   #53844

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   CLDN17 (CLDN17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

5. UBE2C H5.2 gRNA

   Plasmid

   #90934

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human UBE2C

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   UBE2C (Guide Designation H5.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIW086

   Plasmid

   #134132

   Purpose

   Bacterial expression of His-TEV-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-TEV-human Ska1 ; human Ska2
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K183A K184A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

   Plasmid

   #52893

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[2]
   

   Use

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TAL2295

   Plasmid

   #36728

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   APC-TALEN_Right (APC Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. TUBG1 F7.4 gRNA

   Plasmid

   #90930

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TUBG1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TUBG1 (Guide Designation F7.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NUDC D9.4 gRNA

    Plasmid

    #90806

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUDC

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NUDC (Guide Designation D9.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIS1 DICER1 long-mut-let-7

    Plasmid

    #21651

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant let-7 microRNA binding sites

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MIS18BP1 F9.1 gRNA

    Plasmid

    #90768

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MIS18BP1 (Guide Designation F9.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCNL-N1_zyxin

    Plasmid

    #65682

    Purpose

    Expresses Zyxin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    zyxin (Zyx Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. PPP1CB C3.5 gRNA

    Plasmid

    #90845

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP1CB

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP1CB (Guide Designation C3.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCNL-N1_PTS2

    Plasmid

    #65676

    Purpose

    Expresses PTS2-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFGC5941-PacI-ath-STTM773a/b

    Plasmid

    #84141

    Purpose

    target miRNA773a/b for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM773a/b
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)

    Plasmid

    #52906

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[2]+Venus
    

    Use

    Retroviral

    Tags

    L-IFP-F[2]

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pIS1 DICER1 long-mut-all

    Plasmid

    #21653

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated let-7 and miR103/107 microRNA binding sit…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIS1 DICER1 long-mut-miR103/107

    Plasmid

    #21652

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant miR103/107 microRNA binding sites

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge