We narrowed to 77,383 results for: Mycs;

1. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKO (Hyg)

   Plasmid

   #110087

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PHBA02

   Plasmid

   #83536

   Purpose

   Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

   Insert

   pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBY1_FnCpf1cg

   Plasmid

   #104622

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV0661

   Plasmid

   #133476

   Purpose

   Expression in fission yeast from promoter pact1, integrated at ade6 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAMS366-4xCDRE-GFP-PEST

   Plasmid

   #138658

   Purpose

   destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

   Depositor

   Sabrina Büttner

   Article

   Diessl et al Microbial Cell, 2020: in press

   Insert

   4xCDRE-CYC1(PM)-yEGFP3-PEST
   

   Use

   Yeast reporter plasmid

   Tags

   Expression

   Mutation

   Promoter

   CYC1

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIM691

   Plasmid

   #74641

   Purpose

   alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   ymTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBY3_ligD

   Plasmid

   #104624

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   FnCpf1 codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFM689

   Plasmid

   #74635

   Purpose

   alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Inserts

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. YEp181-CUP1-His-Smt3

    Plasmid

    #99536

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMU-AUTr

    Plasmid

    #61204

    Purpose

    Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LC3(ATG8)
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTU-DO-URA

    Plasmid

    #160004

    Purpose

    Low-copy expression vector for K. marxianus, uracil selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    n/a

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST5832

    Plasmid

    #36256

    Depositor

    Carolyn Bertozzi, Jessica Seeliger

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Insert

    lacZ
    

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Modified Hsp60

    Available since

    Nov. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHERRY10

    Plasmid

    #24664

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNRB150

    Plasmid

    #36451

    Depositor

    Douglas Bishop

    Article

    Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

    Insert

    DMC1 (DMC1 Budding Yeast)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIp128-P30-His-POL30

    Plasmid

    #99542

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pI1-MTU-DO-URA

    Plasmid

    #160009

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMA4854

    Plasmid

    #184849

    Purpose

    Coexpression of PhiC31o recombinase and EGFP

    Depositor

    Mikhail Alexeyev

    Article

    Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

    Inserts

    EGFP
    PhiC31o
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and RSV

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p413TEF-Sir2-H364Y

    Plasmid

    #51743

    Purpose

    overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H364Y

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pI6-MTU-DO-URA

    Plasmid

    #160030

    Purpose

    Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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