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We narrowed to 66,707 results for: nin

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Showing: 4901 - 4920 of 66707 results

  7. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  8. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid
    #105447
    Purpose
    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
    Depositor
    Hillel Adesnik
    Publication
    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ChrimsonR-mRuby2-ST
    Tags
    mRuby2 and soma targeting motif
    Expression
    Mammalian
    Mutation
    K176R in Chrimson
    Promoter
    pCAGGS
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105447-plasmid-map-sequence-id-203780

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  9. GST-thr-RAF1-RBD

    Plasmid
    #86033
    Purpose
    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation
    Depositor
    Dominic Esposito
    Insert
    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Contains residues 1-149
    Promoter
    T7
    Available since
    Jan. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86033-plasmid-map-sequence-id-170056

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  12. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Publication
    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    224898-plasmid-map-sequence-id-443622

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  14. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114073-plasmid-map-sequence-id-220529

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  15. CMV-SauriABE8e

    Plasmid
    #189927
    Purpose
    Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.
    Depositor
    David Liu
    Publication
    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SauriABE8e
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A
    Promoter
    CMV
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189927-plasmid-map-sequence-id-371177

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Showing: 4901 - 4920 of 66707 results