We narrowed to 14,443 results for: nts

1. pDGE8

   Plasmid

   #79463

   Purpose

   sgRNA shuttle vector; module 2E

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH49

   Plasmid

   #128211

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

   Plasmid

   #31478

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV HA NLS Y2 K227M T2A BFP
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   F13Y, S111Y, K227M = Y2 nickase "reo" …

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2-GYB

   Plasmid

   #160927

   Purpose

   Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Inserts

   BFP
   sfGFP
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDH3

   Plasmid

   #83789

   Purpose

   Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   CFP
   

   Tags

   CFP

   Expression

   Yeast

   Mutation

   F64L, Y66W, N146I, M153T, V163A, N164H mutations …

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAtCas9_1

   Plasmid

   #91156

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. XE68 APC-GFP in CS2+

   Plasmid

   #16687

   Depositor

   Randall Moon

   Article

   Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

   Insert

   APC-GFP (APC Human)
   

   Use

   Xenopus expression

   Tags

   GFP

   Available Since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pRS416-Aga1p-FAPB2.3.6

   Plasmid

   #158147

   Purpose

   Yeast display reporter for non-display yeast strains (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Expression

   Yeast

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_A0503

   Plasmid

   #91013

   Purpose

   Module A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9
   

   Use

   CRISPR

   Promoter

   CmYLCV

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ITPKA_3xmTurquoise

    Plasmid

    #112961

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    ITPKA
    

    Tags

    3x mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTX198

    Plasmid

    #89262

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpyTag-GlpG

    Plasmid

    #79054

    Purpose

    Expresses SpyTagged GlpG protein

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    SpyTag-GlpG (glpG )
    

    Tags

    6xHis and SpyTag

    Expression

    Bacterial

    Mutation

    GlpG protein employed here corresponds to residue…

    Promoter

    Trc

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2T-CMV-evoAmax-BlastR

    Plasmid

    #152996

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    evoA-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 7iBi89 (rat fibronectin)

    Plasmid

    #14065

    Depositor

    Richard Hynes

    Article

    Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

    Insert

    Rat Fibronectin 7iBi89 (Fn1 Rat)
    

    Expression

    Mammalian

    Mutation

    Insert was cut out of pGEM3 with SalI and SacI an…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pBTK1023 (pDS-InR1)

    Plasmid

    #183130

    Purpose

    Expresses dsRNA targeting the InR1 gene from Apis mellifera

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al Science. 2020 Jan 31;367(6477):573-576.

    Insert

    Fragment of InR1 gene
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHA-ΔN332-Kin1C

    Plasmid

    #31608

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN332 kinesin-1C (Kif5c Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

17. LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

    Plasmid

    #192412

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

    Plasmid

    #192413

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLLX13

    Plasmid

    #79825

    Purpose

    Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

    Depositor

    Cammie Lesser, Stephen Lory

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Yeast and bacteria shuttle vector, contains origi…

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-puro-mTurquoise2-Nuf2

    Plasmid

    #80761

    Purpose

    Express Nuf2 N-terminally labeled with mTurquoise2

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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