We narrowed to 7,424 results for: Tet-on
-
Plasmid#236126PurposePlasmid encoding ADAR2dd attached to PUF-9R under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
ExpressionMammalianMutationE488Q, T501A in ADAR2ddPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)
Plasmid#236146PurposePlasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sitesDepositorInsertNZF-deImmunLink-FRB
ExpressionMammalianMutationN2093W, T2098L in FRBPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Cyr61-rDMR
Plasmid#235692PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assayDepositorInsertCyr61 (Ccn1 Mouse)
UseLuciferaseAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Cyr61-rDMRdeltaTEAD
Plasmid#235693PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Cyr61 lacking Tead-binding motif with luciferase assayDepositorInsertCyr61 (Ccn1 Mouse)
UseLuciferaseAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctgf-rDMR
Plasmid#235696PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Ctgf with luciferase assayDepositorInsertCtgf (Ccn2 Mouse)
UseLuciferaseAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctgf-rDMRdeltaTEAD
Plasmid#235697PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Ctgf lacking Tead-binding motif with luciferase assayDepositorInsertCtgf (Ccn2 Mouse)
UseLuciferaseAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Myc-rDMR
Plasmid#235694PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Myc with luciferase assayDepositorInsertMyc (Myc Mouse)
UseLuciferaseAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y
Plasmid#226349PurposeExpression of Titin V15P substrate with GS tailDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10
Plasmid#226351PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5
Plasmid#226352PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1
Plasmid#226353PurposeExpression of titin (V15P) substrate for 26S proteasomeDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mVprBP pc2953
Plasmid#201698PurposeExpresses of GFP-tagged VprBP in mammalian cellsDepositorAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-mVprBP pc2954
Plasmid#201699PurposeExpresses of mcherry-tagged mVprBP in mammalian cellsDepositorAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
hARL15_32-197
Plasmid#209799PurposeBacterial expression of human ARL15 GTPase domainDepositorAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLA2ScIfmTcIfm
Plasmid#193792PurposeCassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertTwo copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyExpressionBacterialPromoterPLLacO-1 and PLTetO-1Available SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid with onboard mCherry cassette
Plasmid#169735PurposeEqualizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyExpressionMammalianPromoterCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARB366
Plasmid#124049PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB367
Plasmid#124050PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only