169,365 results

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pHR-TRE3G-dCas9-HaloTag

Plasmid

#169448

Purpose

expression of dCas9-HaloTag

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-HaloTag

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONRp5-p2 SspB-mCherry-CShroom3

Plasmid

#170977

Purpose

C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

CShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

SspB and mCherry

Mutation

Isoform X2, deleted aminoacids 1 - 1388

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold2s

Plasmid

#231763

Purpose

Expression of mGold2s in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Mammalian

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-miniAAVR-FLAG

Plasmid

#166717

Purpose

Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

Depositor

Jan Carette

Article

Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

Insert

miniAAVR-FLAG

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Only expressing PKD1-3 of ectodomain

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTECH-MmPylRS(IPYE)

Plasmid

#104073

Purpose

Expression plasmid encoding MmPylRS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MmPylRS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2945

Plasmid

#144421

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLdCN2

Plasmid

#125186

Purpose

Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xFLAG

Plasmid

#129570

Purpose

Empty backbone for FLAG tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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gTBEv5

Plasmid

#220621

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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