We narrowed to 5,124 results for: puc
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pNOC_hfnCas12a-Nlux_crRNA NR1
Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xnr UAS ifgMosaic Donor vector (IG230)
Plasmid#99631PurposeTo clone the 3 ORFs for Gal4 dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)4
Plasmid#127522PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Triple ORF FRT ifgMosaic Donor (IG135)
Plasmid#99626PurposeTo clone the 3 ORFs for FlpO dependent generation of ifgMosaicsDepositorInsertPGK-Neo
UseCre/Lox, Mouse Targeting, and Synthetic BiologyAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Promoter Only Target
Plasmid#127669PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 115 and 117
UseCRISPRTagsHAExpressionMammalianPromoterEF1a and hU6Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBS-4559
Plasmid#249331PurposeExpresses EPEC DarT2(G49D)-nSpCas9(D10A) in mammalian cells and gfp-dropout cassette for cloning sgRNAsDepositorInsertEPEC DarT2(G49D)-nSpCas9(D10A)
Tags3X Flag tagExpressionMammalianMutationDarT2(G49D) and nSpCas9(D10A)Available SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5391
Plasmid#249330PurposeExpresses EPEC DarT2(G49D)-nSpCas9(D10A) and Non-targeting guide for mammalian cellsDepositorInsertEPEC DarT2(G49D)-nSpCas9(D10A)
Tags3X Flag tagExpressionMammalianMutationDarT2(G49D) and nSpCas9(D10A)Available SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5846
Plasmid#249329PurposeExpresses EPEC DarT2(G49D)-nSpCas9(D10A)and sg#3 targeting VEGFA gene in mammalian cellsDepositorInsertEPEC DarT2(G49D)-nSpCas9(D10A)
Tags3X Flag tagExpressionMammalianMutationDarT2(G49D) and nSpCas9(D10A)Available SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-6756
Plasmid#249328PurposeExpresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cellsDepositorInsertEPEC DarT2(G49D)-nSpCas9(D10A)
Tags3X Flag tagExpressionMammalianMutationDarT2(G49D) and nSpCas9(D10A)Available SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-4803
Plasmid#249321PurposeExpresses an sgRNA to target the KanR* site on the engineered E. coli MG1655 strain and contains the Repair Template to replace the KanR* on the genome of E. coli MG1655DepositorInsertsgRNA targeting the KanR* site
ExpressionBacterialMutationN/AAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCBS-5738
Plasmid#249324PurposeTo express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append EditingDepositorInsertEPEC DarT2(DLAA)-nScCas9(D10A)
ExpressionYeastMutationDarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)Available SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(N77)
Plasmid#246056PurposeExpression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before N77) linked through a P2A sequence, under the same pol-III H1 promoterDepositorInsertSauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-N77
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41)
Plasmid#246055PurposeExpression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before D41) linked through a P2A sequence, under the same pol-III H1 promoterDepositorInsertSauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-D41
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSOX17-T2A-dTom-PGK-Neo
Plasmid#223190PurposeTomato reporter plasmid targeting the human SOX17 geneDepositorInsertSOX17-T2A-dTomato-PGK-Neo-SOX17 (SOX17 Human)
UseCre/Lox; Human targetingAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-LMNB1
Plasmid#227328PurposeDonor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)DepositorInsertLMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Puro-TP53
Plasmid#227319PurposeDonor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)DepositorInsertTP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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