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pET28 His6-mEGFP-D4H

Plasmid

#226373

Purpose

Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4H (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF1A

Plasmid

#172206

Purpose

Expresses KIF1A tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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SNAPtag-PTS1

Plasmid

#182883

Purpose

Express SNAPtag-PTS1 in mammalian cells. SNAPtag targets to peroxisomes

Depositor

Pekka Katajisto

Article

Bui et al Nat Commun. 2025 Apr 26;16(1):3932. doi:

Insert

SNAPtag-PTS1

Use

Lentiviral

Tags

SNAPtag

Expression

Mammalian

Mutation

no

Promoter

CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1_BRCT W706RW815R

Plasmid

#250355

Purpose

Expresses mutant tandem BRCT domain from MCPH1 fused to eGFP. Serves as control.

Depositor

Tuncay Baubec

Article

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Mutation

W706R and W815R

Promoter

CAGGS

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-HaloTag7-CHMP4A

Plasmid

#184895

Purpose

Stable expression of HaloTag7-CHMP4A in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

CHMP4A (CHMP4A Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato-Mito-7

Plasmid

#58115

Purpose

Localization: Mitochondria, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

Mito (COX8A Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pYJA5

Plasmid

#217778

Purpose

The empty vector for quadruple sgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Puromycin resistance gene, T2A, and TagBFP

Expression

Mammalian

Promoter

PGK, CMV, U6

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAKTaq

Plasmid

#25712

Purpose

Bacterial expression of Taq polymerase

Depositor

David Engelke

Article

Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

Insert

Taq polymerase

Expression

Bacterial

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR1c-V5/HIS

Plasmid

#201106

Purpose

expression of human FGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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hMTP-flag

Plasmid

#138335

Purpose

Expresses MTP

Depositor

Mahmood Hussain

Article

Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

Insert

MTTP (MTTP Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cre

Plasmid

#198281

Purpose

Expresses Cre in mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Cre

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pOSIP-KL-mcherry

Plasmid

#116947

Purpose

Integrating mCherry into lambda attB in E. coli chromosome.

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

mcherry

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV/cmGAD67 GFP WPRE SV40pA

Plasmid

#245935

Purpose

GABAergic neuron-specific cmGAD67 promoter

Depositor

Hirokazu Hirai

Insert

Mouse GAD67 promoter, Region ii to Etss (Transcriptional Start Site in Exon1)

Use

AAV

Tags

GFP

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8

Plasmid

#11817

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 (CASP8 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF15_P2A_Hygro_Barcode

Plasmid

#120501

Purpose

Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF15 (KLF15 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJRH051

Plasmid

#171625

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BsmBI-Destination
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2188-PrARG8-GFP::URA3

Plasmid

#216512

Purpose

Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrARG8-yeGFP

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

ARG8

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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