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pCAG_eGCaMP(plus)

Plasmid

#201147

Purpose

expresses eGCaMP+ in mammalian cells

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP+

Expression

Mammalian

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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p346_DG

Plasmid

#234676

Purpose

Golden gate domesticated pET28b with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGCS-C6(eGFP)

Plasmid

#85729

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

EGFP

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA EYFP

Plasmid

#10825

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

EYFP

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pKmK.C1

Plasmid

#125059

Purpose

Storage plasmid for K.marxianus centromere

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS7/CenD

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.C2

Plasmid

#125060

Purpose

Storage plasmid for K.marxianus centromere

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS/CEN5

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.C3

Plasmid

#125061

Purpose

Storage plasmid for K.marxianus centromere

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS/CEN6

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

Plasmid

#216665

Purpose

Expresses Gal4 inducible BFP, with hygromycin resistance

Depositor

Leonardo Morsut

Inserts

BFP
HygromycinR

Use

Lentiviral

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST26-NAT1-C-HA

Plasmid

#195178

Purpose

Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

Depositor

Stephanie Roessler

Insert

NAT1 (NAT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKmK.C4

Plasmid

#125062

Purpose

Storage plasmid for K.marxianus ARS

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KmARS2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGCS-C5(GST)

Plasmid

#85728

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

GST

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Expression

Mammalian

Promoter

mini-CAG

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp12

Plasmid

#72732

Purpose

pFUGW-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

CMVp-GFP

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pStA1BZ

Plasmid

#114164

Purpose

Start-Stop Assembly Level 1BZ plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA1BC

Plasmid

#114165

Purpose

Start-Stop Assembly Level 1BC plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_CAG_Gag

Plasmid

#212634

Purpose

Lentivirus vector to express MLV Gag

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGRNA2e

Plasmid

#169782

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGRNA1

Plasmid

#169778

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRainbow-DONOR1

Plasmid

#75398

Purpose

CRISPRainbow-DONOR1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Cm(R)-CcdB

Use

CRISPR

Tags

no

Promoter

Bacteria

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCYC2

Plasmid

#43970

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

CYC1

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pRSET-jAspSnFR3

Plasmid

#203458

Purpose

jAspSnFR3 in T7 bacterial expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKJ1267 (aka pAC-alphaGal4)

Plasmid

#21869

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pGRNA3e

Plasmid

#169783

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGRNA2

Plasmid

#169779

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pLV_EF1a_Gag

Plasmid

#212638

Purpose

Lentivirus vector to express MLV Gag

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHaloTag-PS

Plasmid

#86626

Purpose

Expresses Akt pseudosubstrate tagged with a HaloTag

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag-PS(RA)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTGD

Plasmid

#88894

Purpose

Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli

Depositor

Keith Tyo

Article

Love et al Methods Mol Biol. 2019;1927:37-45. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCSaE500

Plasmid

#53182

Purpose

Inducible expression of E gene which produces a lysis protein that induces autolysis.

Depositor

Lingchong You

Article

Tanouchi et al Mol Syst Biol. 2012;8:626. doi: 10.1038/

Insert

pCSaE500

Promoter

ampR-PampC cassette

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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