We narrowed to 9,370 results for: BLI

1. pENTRY4_HuB

   Plasmid

   #65752

   Purpose

   entry vector with HuB

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Expression

   Mammalian

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. hSyn-Aion-ts-mScarlet-ER

   Plasmid

   #192577

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   ts-mScarlet-ER

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV Cnep1r1-V5

   Plasmid

   #175133

   Purpose

   Lentiviral expression of mouse Cnep1r1-V5

   Depositor

   Larry Gerace

   Insert

   Cnep1r1 (Cnep1r1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4P7A

   Plasmid

   #61954

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MLH1:JMC01L-F09:C205690 (MLH1 Human)
   

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   March 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pHyg-PCUP1-AID*(N)

   Plasmid

   #99529

   Purpose

   N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Insert

   IAA17(71-117) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Promoter

   CUP1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307-CTSL-G418

   Plasmid

   #158468

   Purpose

   Constitutive lentiviral expression of human CTSL.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   CTSL (CTSL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ENO3

   Plasmid

   #39072

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ENO3 (ENO3 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. isoform PKC Zeta 2 (JCE863)

   Plasmid

   #182028

   Purpose

   yeast expression of mouse PKC Zeta isoform 2 with yeast PKC1 promoter and ADH1 terminator

   Depositor

   Juan Carlos Igual

   Insert

   PKC Zeta isoform 2
   

   Expression

   Mammalian

   Promoter

   yeast PKC1 promoter (-600 to -1)

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

   Plasmid

   #44081

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Available Since

   April 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV Ef1a-mRuby3-WPRE

    Plasmid

    #135427

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Insert

    mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTre-Tight-luciferase-Hottip (522)

    Plasmid

    #65490

    Purpose

    positive control for lncRNA expression assay

    Depositor

    Rory Johnson, Roderic Guigo

    Insert

    Hottip (HOTTIP Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpot-Tag-Lamin

    Plasmid

    #117062

    Purpose

    For mammalian expression of lamin B1 with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    Lamin B1 (LMNB1 Human)
    

    Tags

    Spot-Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTRY4_HuD

    Plasmid

    #65754

    Purpose

    entry vector with HuD

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Expression

    Mammalian

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid

    #104419

    Purpose

    transient expression of dCas9-dp300 fusion protein

    Depositor

    Stephan Beck

    Insert

    dp300
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    S1396R-S1397R

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIC84

    Plasmid

    #63062

    Purpose

    Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    hCENP-H, IMAGE clone 3451767 (CENPH Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBACgus4x/Parkin

    Plasmid

    #40385

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Parkin (PRKN Human)
    

    Use

    Baculovirus

    Mutation

    WT

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

    Plasmid

    #44093

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKG402

    Plasmid

    #69760

    Purpose

    Retroviral expression of CENP-T aa 256-561

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 256-561 (CENPT Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid

    #50417

    Purpose

    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.

    Depositor

    Andrew Scharenberg

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBluescript alpha-SSeCKS

    Plasmid

    #61295

    Purpose

    contains rat alpha SSeCKS cDNA

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Expression

    Bacterial

    Mutation

    cDNA with 1kb of 3' UTR and 150bp of 5'…

    Promoter

    T7

    Available Since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only