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We narrowed to 63,680 results for: cloning

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Showing: 4961 - 4980 of 63680 results

  2. AICSDP-24:MYH10-mEGFP

    Plasmid
    #87428
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYH10 Homology Arms with mEGFP-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87428-plasmid-map-sequence-id-177429

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  3. pTetON-DeAct-GS1

    Plasmid
    #89454
    Purpose
    Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89454-plasmid-map-sequence-id-176821

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  9. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid
    #84247
    Purpose
    Expresses direct fusion VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84247-plasmid-map-sequence-id-160890

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  14. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Publication
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. pFRT-DestFLAGHA_RNAseH1

    Plasmid
    #65783
    Purpose
    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65783-plasmid-map-sequence-id-151430

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  18. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62329-plasmid-map-sequence-id-296399

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Showing: 4961 - 4980 of 63680 results