We narrowed to 14,443 results for: nts

1. LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ

   Plasmid

   #192432

   Purpose

   To act as a 2-input state of the NOR gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-B3RT-Rluc-B3RT-FRT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML104-kanR1136

   Plasmid

   #162040

   Purpose

   Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

   Insert

   kanR1136 guide
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE5

   Plasmid

   #79461

   Purpose

   sgRNA shuttle vector; module 1

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG5-mERRbeta-short

   Plasmid

   #52188

   Purpose

   Expresses mouse ERRbeta short form splice variant in mammalian cells

   Depositor

   Rebecca Riggins

   Article

   Heckler et al Cell Cycle. 2014 Dec 12:0.

   Insert

   Estrogen-related receptor beta (Esrrb Mouse)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pUPD2 SV40-AcrIIA4 (GB3333)

   Plasmid

   #160605

   Purpose

   SV40-AcrIIA4 codon optimized for N.benthamiana

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE662

   Plasmid

   #153260

   Purpose

   M6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE664

   Plasmid

   #153262

   Purpose

   M7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGE659

   Plasmid

   #153257

   Purpose

   M4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE661

   Plasmid

   #153259

   Purpose

   M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRANS_263

    Plasmid

    #91127

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3_AKAR5Δ

    Plasmid

    #119912

    Purpose

    Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    AKAR5Δ
    

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET:GCAT:GFP

    Plasmid

    #60736

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. KG#470

    Plasmid

    #110930

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FusionRed-BaxAlpha-C-15

    Plasmid

    #56101

    Purpose

    Localization: , Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    BaxAlpha
    

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pFLIPglu-3.2mDelta13

    Plasmid

    #12992

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A, S112A in binding protein

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pHJ18

    Plasmid

    #48357

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFLIPglu-170nDelta13

    Plasmid

    #12989

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    WT binding protein (no F16A)

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pDest_pK7WG2_pRPS5A_CTP OleGFP

    Plasmid

    #171723

    Purpose

    ptpTALECD vector assembly, Destination vector;

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
    

    Expression

    Plant

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. E2_pENTR_R4R3_T CTP

    Plasmid

    #171736

    Purpose

    ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEE389

    Plasmid

    #149287

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only