We narrowed to 7,454 results for: ski

1. MCP-NMS

   Plasmid

   #210705

   Purpose

   This plasmid express EF1a promoter driven MCP-NMS transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-flex-GFP-shRNA-GluN1

   Plasmid

   #182502

   Purpose

   Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
   

   Use

   AAV, Cre/Lox, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPN089

   Plasmid

   #91614

   Purpose

   Express sgRNA targeting human FURIN

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   FURIN (FURIN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV.CAG.SF-iGluSnFR.S72A

   Plasmid

   #106200

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: S72A

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Src-SH2

   Plasmid

   #46510

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Src (SRC Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 142-252

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Basigin-isoform2-CD4d3+4-bio

   Plasmid

   #73129

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Basigin isoform 2 (BSG Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hSyn-DIO-mCReP

   Plasmid

   #167503

   Purpose

   Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   Ppp1r15b (Ppp1r15b Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIB-VP64 (B1016)

   Plasmid

   #92037

   Purpose

   Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1-VP64 (CIB1 Mustard Weed)
   

   Use

   Tags

   Fusion of plant CIB1 with VP64 activation domain.…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-CAG-F14F15S-Switch

   Plasmid

   #99733

   Purpose

   Flp-based FLEX reporter (from RFP to GFP)

   Depositor

   Ian Wickersham

   Article

   Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

   Insert

   TagRFP and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/NDUFS2/myc-His

    Plasmid

    #127486

    Purpose

    Expresses myc-tagged NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-DIO-Casp3-TEV

    Plasmid

    #183766

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNL-CEF-IgA1-IRES-GFP

    Plasmid

    #187007

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-PE

    Plasmid

    #162796

    Purpose

    All-in-one prime editor piggyBac transposon

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCTIP2-N174

    Plasmid

    #60858

    Purpose

    Expresses CTIP2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    CTIP2 isoform b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/NDUFS7/myc-His

    Plasmid

    #127488

    Purpose

    Expresses myc-tagged NDUFS7 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS7 (NDUFS7 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TurboID-13x-EV

    Plasmid

    #215086

    Purpose

    Expresses TurboID-13x[linker] in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    13x [linker]

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSynapsin.SF-iGluSnFR.A184V

    Plasmid

    #106175

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184V

    Promoter

    hSynapsin

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE HA Chk2 KD, dominant negative chk2

    Plasmid

    #41902

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D347A

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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