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pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES_Tomato

Plasmid

#107229

Purpose

Tomato expressing plasmid

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA1

Plasmid

#166913

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA2

Plasmid

#166914

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-MARK3

Plasmid

#107235

Purpose

Lentiviral gene expression vector for phospho-MARK3 expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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p323.hSyn.Halo-mFMRP.Wpre

Plasmid

#185909

Purpose

Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

Depositor

Young J Yoon

Article

Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

Insert

HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)

Use

Lentiviral

Tags

HaloTag

Promoter

hSyn (human Synapsin promoter)

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Ezh2

Plasmid

#118587

Purpose

Expresses Ezh2 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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