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pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Expression

Worm

Promoter

Promoter Ultra Pan Neuronal

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCM1.56

Plasmid

#17251

Depositor

Geraldine Seydoux

Article

Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

Insert

L3787 promoter NO ATG in pDONRP4P1R

Use

Gateway

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Tags

V5; mCherry

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJH2217

Plasmid

#179251

Purpose

Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pnmr-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDO001

Plasmid

#198828

Purpose

Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGC479

Plasmid

#42601

Depositor

Jane Hubbard

Article

Korta et al Development. 2012 Mar;139(5):859-70. doi

Insert

daf-15 (daf-15 Nematode)

Use

RNAi

Promoter

T7

Available Since

April 19, 2013

Availability

Academic Institutions and Nonprofits only
pDR3.10

Plasmid

#19367

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

msp-142 ORF+3' in pDONR201

Expression

Worm

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pSH1

Plasmid

#90295

Purpose

Expresses the fat-7 gene in C. elegans intestine

Depositor

Anne Brunet

Article

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

fat-7 (fat-7 Nematode)

Tags

No

Expression

Worm

Promoter

ges-1

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Article

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Tags

GFP

Expression

Worm

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2838

Plasmid

#87759

Purpose

Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmyo-3-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmyo-3

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAS1-odr-4P::his-24::mNeptune

Plasmid

#124342

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

odr-4, mNeptune (odr-4 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1846

Plasmid

#154326

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP^SEC^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMS051

Plasmid

#137025

Purpose

SapTrap mScarlet-I-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized mScarlet

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized mScarlet

Expression

Worm

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRG5273Neo

Plasmid

#26388

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pWZ194

Plasmid

#163640

Purpose

rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)

Use

CRISPR

Tags

2xmKate2 and GFP

Expression

Worm

Promoter

rps-27

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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