Addgene

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pJA254

Plasmid

#21507

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5::GFP-F64LS65T::(Gly)5Ala

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGC219

Plasmid

#19687

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phlh-12

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Article

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCZGY2838

Plasmid

#87759

Purpose

Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmyo-3-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRG5271Neo

Plasmid

#26387

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Aug 22. ():.

Type

Empty backbone

Use

Tags

Expression

Bacterial and Worm

Mutation

Promoter

Available since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Pu2-3::tag::sl1(G16C)

Plasmid

#79002

Purpose

Expression of exogenous SL1 in multiple tissues of C. elegans

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Use

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

F08G2.17

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJH3185

Plasmid

#132367

Purpose

Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.04

Plasmid

#171209

Purpose

bacterial expression of MBP-tagged PGL-3

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

pgl-3 (pgl-3 Nematode)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pJRM3 - [BAGp | mScarlet | tbb-2 UTR]

Plasmid

#204616

Purpose

mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[BAGp | wrmScarlet | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJRM4 - [BAGp | gfp | tbb-2 UTR]

Plasmid

#204617

Purpose

gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[BAGp | gfp | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBT3035

Plasmid

#122543

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_lmn-1::CemOrange2 (lmn-1 Nematode, Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPD133.47

Plasmid

#27510

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pmyo-3::cfp::lacZ::unc-54_3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Use

Tags

V5; mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Use

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Promoter

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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