Addgene

We narrowed to 880 results for: fkbp

Showing: 721 - 750 of 880 results

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pLX307_CdTag-HA_EmptyVector

Plasmid

#221467

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLX302_CdTag-HA_EmptyVector

Plasmid

#221465

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP_CdTag-HA

Plasmid

#221492

Purpose

GFP lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pC4EN-F1-GAFm

Plasmid

#39870

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein GAF domain (mutated)

Use

Tags

FKBP

Expression

Mammalian

Mutation

F165Y, D232Y, W308R

Promoter

CMV

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
YIplac204-pOst1-ESCargo(Crimson)

Plasmid

#140154

Purpose

Expresses a vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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CF-Ins54p

Plasmid

#20155

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (INP54 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Stop codon at position 331.

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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PX854

Plasmid

#62886

Purpose

C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa573-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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YF-Tiam1

Plasmid

#20154

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Tiam1 (TIAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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CF-iSH

Plasmid

#20159

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-p85 (Pik3r2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pINT-PuroDDEYFP

Plasmid

#31442

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

enhanced yellow fluorescent protein

Use

Phic31/attb

Tags

FKBP L106P

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pERB109

Plasmid

#58280

Purpose

constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pERB130

Plasmid

#58284

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pERB127

Plasmid

#58283

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pERB160

Plasmid

#58285

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pERB122

Plasmid

#58282

Purpose

const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
GFP
Puromycin resistance
inducible mCherry-FRB

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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iXFGFR4k

Plasmid

#31260

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR4-ICD-FKBP (fgfr4-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pERB171

Plasmid

#58289

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1^485-718
FKBP-targeted miRNA

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pERB170

Plasmid

#58288

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-584

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX856

Plasmid

#62888

Purpose

C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS, NLS SV40, and VP64

Expression

Mammalian

Mutation

Asparagine 863 to Alanine (N863A)

Promoter

CBh

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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CF-Inp(D281A)

Plasmid

#20156

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (D281A) (INP54 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Stop codon at position 331. D281A mutation.

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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CF-PIPK

Plasmid

#20157

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-PIPK (Pip5k1c Mouse)

Use

Tags

Expression

Mammalian

Mutation

C-terminus (amino acids 636-661)truncated, R445E,…

Promoter

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRB

Plasmid

#104475

Purpose

Plasmid carrying FRB with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRB (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry Tshld-GFP

Plasmid

#53211

Purpose

pEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld-GFP

Use

Tags

EGFP, Flag, and HA

Expression

Mutation

Promoter

none

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB-polySH33

Plasmid

#175233

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac204-pOst1-ESCargo

Plasmid

#115420

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescriptKS(-)-FRBx2

Plasmid

#104476

Purpose

Plasmid carrying FRBx2 with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRBx2 (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge