-
-
pSH1/M-Fv'-Fvls-E
Plasmid#15273DepositorInsertFKBP12(V36) (FKBP1A Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationFKBP12 F36-->V 1st FKBP uses "wobbled&qu…PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationE192K_D193KPromoterT7Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_I48N
Plasmid#214323PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationI48NPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_mouse-Pro-IL-18_humanized-triple-mutant
Plasmid#214339PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Mouse)
UseTagsHis-TEVExpressionBacterialMutationENGD29DDEN_N36Y_H41EPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_LES33AAA
Plasmid#214326PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationLES33AAAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DDEN29ENGD
Plasmid#214325PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDDEN29ENGDPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_V47N_I48N
Plasmid#214324PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationV47N_I48NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_rat
Plasmid#214333PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Rat)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Pansio
Plasmid#214441PurposeBxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Keppel
Plasmid#214444PurposeBxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA4.721-Olônne
Plasmid#214443PurposeBxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.DepositorInsert2xFKBP12-Casp9
UseHdr donorTagsP2A-BsdExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP/golgin-160
Plasmid#180630PurposeExpression of WT golgin-160DepositorInsertgolgin-160 (GOLGA3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-FLIP1500
Plasmid#16016DepositorInsertFLIP (CFLAR Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET26b-SET
Plasmid#8822DepositorInsertSET (SET Human)
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTU387
Plasmid#178981PurposepACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR arrayDepositorInsertCRISPR array from Candidatus Scalindua brodae
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingTagsExpressionMutationPromotermNanogAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to scFv (anti-spike)
Plasmid#186421PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2DepositorInsertdCas9-scFv fusion (anti-SARS-CoV-2 spike)
UseCRISPRTags6HisExpressionMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_V47N_I48N
Plasmid#214319PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertIL18 (IL18 Human)
UseRetroviralTagsFlag and MycExpressionMutationV47N_I48NPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only
