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Showing: 481 - 500 of 672 results

  2. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

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  7. pAcBac1-Ma-sfGFP WT

    Plasmid
    #197567
    Purpose
    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    V5-His6
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    197567-plasmid-map-sequence-id-389420

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  12. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid
    #185299
    Purpose
    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_shuffle_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185299-plasmid-map-sequence-id-363926

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  13. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid
    #185298
    Purpose
    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    PvLEA4_repeats_k3_26
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185298-plasmid-map-sequence-id-363925

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  14. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid
    #185303
    Purpose
    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_mutant_3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185303-plasmid-map-sequence-id-363934

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  15. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

    Plasmid
    #185302
    Purpose
    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    PvLEA4_repeats_k5_1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185302-plasmid-map-sequence-id-363930

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  16. pvLEA22mer_mutant_10_mCh-SspB (pBS1078)

    Plasmid
    #185301
    Purpose
    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    pvLEA22mer_mutant_10
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185301-plasmid-map-sequence-id-363929

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  18. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  20. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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Showing: 481 - 500 of 672 results