We narrowed to 3,877 results for: SPL

1. pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

   Plasmid

   #107135

   Purpose

   cGAL-C driver under the control of C. elegans myo-2 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::gp41-1-C-intein::cGAL(AD)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans myo-2 promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CTE-Luc

   Plasmid

   #60881

   Purpose

   Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

   Depositor

   Vinay Nandicoori

   Article

   Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

   Insert

   Luc-CTE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)

   Plasmid

   #107134

   Purpose

   cGAL-N driver under the control of C. elegans myo-2 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::cGAL(DBD)::gp41-1-N-intein
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans myo-2 promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-Top-Toehold-mCherry

   Plasmid

   #87318

   Purpose

   Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-P70a-Top-T~Bottom-T

   Plasmid

   #87315

   Purpose

   Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system (promoter control)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHYDuet93

   Plasmid

   #42303

   Depositor

   Hideo Iwai

   Article

   Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

   Insert

   NpuDnaEdC6
   

   Use

   Tags

   H6-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSuper-TatSF1 GH

   Plasmid

   #31120

   Depositor

   Mariano Garcia-Blanco

   Article

   Miller et al PLoS One. 2009 . 4(5):e5710.

   Insert

   TatSF1 shRNA (HTATSF1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pSuper-TatSF1 AB

   Plasmid

   #31119

   Depositor

   Mariano Garcia-Blanco

   Article

   Miller et al PLoS One. 2009 . 4(5):e5710.

   Insert

   TatSF1 shRNA (HTATSF1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

9. GST-beta catenin tag

   Plasmid

   #186422

   Purpose

   Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   GST-beta catenin tag
   

   Use

   Tags

   6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-TRE3G-NODALvar-matMYC

    Plasmid

    #115641

    Purpose

    For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL splice variant (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. NODALvar-MYC-DYK-N328A

    Plasmid

    #115246

    Purpose

    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tag

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL splice variant (NODAL Human)
    

    Use

    Tags

    MYC-DYK

    Expression

    Mammalian

    Mutation

    N328A

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIR196

    Plasmid

    #64811

    Purpose

    SPL9 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    SPL9 (SPL9 Mustard Weed)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHYBAD94

    Plasmid

    #42305

    Depositor

    Hideo Iwai

    Article

    Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

    Insert

    NpuDnaEC6
    

    Use

    Tags

    GB1-H6

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA RBD-GFP11

    Plasmid

    #133069

    Purpose

    Expresses RBD domain with tripartite split GFP11 (M4)

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    RBD (Rhotekin-Rho Binding Domain)
    

    Use

    Tags

    GFP11 (tripartite split-GFP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pComb3XTT

    Plasmid

    #63891

    Purpose

    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    6xHis, HA tag, and geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA GFP10-ZIP

    Plasmid

    #182241

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP
    

    Use

    Tags

    split-GFP10 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA ZIP-GFP11

    Plasmid

    #182242

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP11

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    ZIP-GFP11
    

    Use

    Tags

    split-GFP11 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid

    #115775

    Purpose

    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    

    Use

    Tags

    EGFP; dsReD

    Expression

    Mammalian

    Mutation

    gp140 uncleaved (1190-91aa, KR > TG; truncated…

    Promoter

    HIV-1 (LTR)

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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