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p38.pA.OFP.mCMV.hPGK.G5-Flag-NGFR.WPRE

Plasmid

#226761

Purpose

5G repeat, NGFR in p20

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

ngfr (NGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-psbA2 point mutation

Plasmid

#182929

Purpose

point mutation on psbA2 by CRISPR in Synechocystis 6803

Depositor

Himadri Pakrasi

Article

Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

Inserts

ddcpf1
gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
psbA

Use

CRISPR

Tags

Expression

Mutation

S264A, and silent mutation to remove PAM site

Promoter

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET151-hUCH37 (ISF2)

Plasmid

#61930

Purpose

Expression of hUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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PB-SAM-Dest

Plasmid

#102563

Purpose

Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

N/A

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMCSF-R-luc

Plasmid

#12420

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pmCherry-C1_SEPT9 i1

Plasmid

#71622

Purpose

mammalian expression of human SEPT9 with an mCherry fusion

Depositor

Elias Spiliotis

Article

Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

Insert

Septin 9 (SEPTIN9 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBTK519

Plasmid

#110603

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TRAIL-R1

Plasmid

#61382

Purpose

Expresses HA tagged TRAIL receptor 1

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 1

Use

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R1 leader peptide (aa1-23) replaced with TN…

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pBTK501

Plasmid

#110602

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK510

Plasmid

#110611

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK564

Plasmid

#110613

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK520

Plasmid

#110604

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK509

Plasmid

#110610

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT.000

Plasmid

#119559

Purpose

Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Dectin1A-C-10

Plasmid

#55290

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Dectin1A (CLEC7A Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBTK563

Plasmid

#110612

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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