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Showing: 481 - 500 of 1532 results

  1. THI VP64 pALD4 T3

    Plasmid
    #161484
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T3_gRNA_MS2
    Depositor
    Diethard Mattanovich, Brigitte Gasser
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (targeting T3 of pTHI11 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161484-plasmid-map-sequence-id-324354

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  2. VanR - F165V

    Plasmid
    #158965
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165V
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158965-plasmid-map-sequence-id-319279

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  3. VanR - F165R

    Plasmid
    #158966
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165R
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158966-plasmid-map-sequence-id-319292

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  7. ampl43

    Plasmid
    #161830
    Purpose
    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.
    Depositor
    Saeed Tavazoie
    Article
    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    161830-plasmid-map-sequence-id-318315

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  9. SZ_EC_02

    Plasmid
    #155341
    Purpose
    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
    Depositor
    Jens Nielsen
    Article
    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:
    Inserts
    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    Use
    Easycloneyali insert vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    gapdh, pyk1, and tef1
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    155341-plasmid-map-sequence-id-316921

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  10. pCfB2793

    Plasmid
    #64043
    Purpose
    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
    Depositor
    Irina Borodina, Jerome Maury
    Article
    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi
    Insert
    GFP
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter and none
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    64043-plasmid-map-sequence-id-149101

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  12. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  15. pCAS9c

    Plasmid
    #141250
    Purpose
    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141250-plasmid-map-sequence-id-308875

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  17. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  18. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid
    #202585
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …
    Promoter
    Available since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    202585-plasmid-map-sequence-id-414431

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  19. pcDNA3.1-mCherry-CyrCR1.miR16

    Plasmid
    #128747
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128747-plasmid-map-sequence-id-254135

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Showing: 481 - 500 of 1532 results