We narrowed to 59,251 results for: cloning

1. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   lacZ

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p663-UBC-PM-TurboID-V5_IDG-K

   Plasmid

   #214455

   Purpose

   Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   PM-TurboID-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pStart-K

   Plasmid

   #20346

   Depositor

   Mario Capecchi

   Article

   Wu et al Nat Protoc. 2008 . 3(6):1056-76.

   Type

   Empty backbone

   Use

   Mouse Targeting; Gateway cloning

   Available Since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-PM-miniTurbo-V5_IDG-K

   Plasmid

   #214454

   Purpose

   Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   PM-miniTurbo-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBPcyto-TEV-BseRI

   Plasmid

   #157739

   Purpose

   empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Tags

   His10 + MBP (cytoplasmic expression; no signal se…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGW_SM_BamHI

   Plasmid

   #49315

   Purpose

   Subcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insert

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD(Gib)_3'omega

   Plasmid

   #223870

   Purpose

   4G-cloning donor for 3'-Gibson overhang, omega-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'omega sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD(Nt)_His6-TEV

   Plasmid

   #223872

   Purpose

   4G-cloning donor for N-terminal His6-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD(Nt)_His6-3C

   Plasmid

   #223873

   Purpose

   4G-cloning donor for N-terminal His6-3C tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-3C
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD(Nt)_His6-FLAG3

    Plasmid

    #223874

    Purpose

    4G-cloning donor for N-terminal His6-FLAG3 tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-FLAG3
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD(P+T)_ph

    Plasmid

    #223857

    Purpose

    4G-cloning donor for insect promoter and terminator, no tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    polyhedrin promoter and SV40 polyA
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD(P+T)_CMV

    Plasmid

    #223860

    Purpose

    4G-cloning donor for mammalian promoter and terminator, no tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    CMV promoter and bGH polyA
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD(P+T)_T7

    Plasmid

    #223854

    Purpose

    4G-cloning donor for bacterial promoter and terminator, no tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    T7 promoter and terminator
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD(Nt)_His6-SUMO

    Plasmid

    #223879

    Purpose

    4G-cloning donor for N-terminal His6-SUMO tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-SUMO
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD(Nt)_GFP-TEV

    Plasmid

    #223881

    Purpose

    4G-cloning donor for N-terminal GFP-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    GFP-TEV
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD(Nt)_TS-3C

    Plasmid

    #223883

    Purpose

    4G-cloning donor for N-terminal TwinStrep-3C tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    TwinStrep-3C
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD(Nt)_GFP-3C

    Plasmid

    #223882

    Purpose

    4G-cloning donor for N-terminal GFP-3C tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    GFP-3C
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD(Ct)_3C-GFP

    Plasmid

    #223888

    Purpose

    4G-cloning donor for C-terminal 3C-GFP tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    3C-GFP
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD(Ct)_3C-His8

    Plasmid

    #223887

    Purpose

    4G-cloning donor for C-terminal 3C-His8 tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    3C-His8
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD(Ct)_CPD-His10

    Plasmid

    #223890

    Purpose

    4G-cloning donor for C-terminal CPD-His10 tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    CPD-His10
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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