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Showing: 481 - 500 of 512 results

  3. t1-405(del37/GAG)

    Plasmid
    #166129
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del37/GAG) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405, with 37 aa deletion in the F1-loop which…
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166129-plasmid-map-sequence-id-330258

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  4. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

    Plasmid
    #120407
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with mutations …
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120407-plasmid-map-sequence-id-232461

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  6. t1-405(del30)

    Plasmid
    #166128
    Purpose
    Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del30) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, with 30 amino acid deletion in th…
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166128-plasmid-map-sequence-id-330260

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  7. t1-405(T144E,T150E)

    Plasmid
    #166130
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    HuT1head1-405(T144E,T150E) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, mutations T144E and T150E
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166130-plasmid-map-sequence-id-330250

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  8. t1-405(151-154AAAA)

    Plasmid
    #166131
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(151-154AAAA) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405 only. Contains 4 amino acid substitutions…
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166131-plasmid-map-sequence-id-330282

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  10. pAraTMDN

    Plasmid
    #47515
    Purpose
    Contains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assay
    Depositor
    Bryan Berger
    Article
    Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836
    Insert
    Integrin alpha2B L980A TM-CYTO
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Trc
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47515-plasmid-map-sequence-id-67699

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Showing: 481 - 500 of 512 results