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pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Tags

Expression

Mutation

Promoter

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR-CDKN2A-Ex2-CMV-EGFP

Plasmid

#110734

Purpose

Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

Plasmid

#110735

Purpose

Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

H2B-mOrange2

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGL410-BRN2p

Plasmid

#110733

Purpose

Luciferase reporter for the human BRN2 promoter

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRN2 promoter luciferase (POU3F2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-INK4A-zsGreen

Plasmid

#110728

Purpose

Mammalian Expression of p16INK4A IRES zsGreen

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES zsGreen (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-ARF-zsGreen

Plasmid

#110729

Purpose

Mammalian Expression of p14ARF IRES zsGreen

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p14ARF IRES zsGreen (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-INK4A-mOrange2

Plasmid

#110730

Purpose

Mammalian Expression of p16INK4A IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES H2B-mOrange2 (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-ARF-mOrange2

Plasmid

#110731

Purpose

Mammalian Expression of p14ARF IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p14ARF IRES H2B-mOrange2 (CDKN2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-BRAFV600E-mOrange2

Plasmid

#110732

Purpose

Mammalian Expression of BRAFV600E IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRAFV600E IRES H2B-mOrange2 (BRAF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

BRAF V600E

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEN35-CDKN2A-Ex2-R

Plasmid

#110737

Purpose

Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

CDKN2A gRNA (CDKN2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEN35-CDKN2A-Ex2-L

Plasmid

#110736

Purpose

Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

CDKN2A gRNA (CDKN2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pNtCp-rbcL-accD

Plasmid

#52469

Purpose

Contains rbcL and accD DNA regions for double homologous recombination useful in Nicotiana tabacum Cp transformation

Depositor

Anastasios Melis

Insert

rbcL-accd Nicotiana tabacum Cp DNA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM81975

Plasmid

#219515

Purpose

Silencing of Nb RT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Nb RT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM81987

Plasmid

#219516

Purpose

Silencing of Nb RT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Nb RT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM81963

Plasmid

#219514

Purpose

Silencing of Nb F3'5'H

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Nb F3'5'H

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-tracrRNA-PMtU6-26

Plasmid

#209191

Purpose

Cloning of sgRNAs for expression in plants

Depositor

Christian Staehelin

Insert

MtU6-26 SnRNA promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-tracrRNA-PMtU6-1

Plasmid

#209190

Purpose

Cloning of sgRNAs for expression in plants

Depositor

Christian Staehelin

Insert

MtU6-1 SnRNA promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pISV-CRISPR/Cas9PGmUbi

Plasmid

#209189

Purpose

Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

Depositor

Christian Staehelin

Inserts

Cas9
DsRed(deltaB)

Use

Tags

NLS

Expression

Plant

Mutation

missing BsaI site and plant-codon optimized with …

Promoter

CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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XE9 Xwnt-8 CSKA

Plasmid

#16866

Depositor

Randall Moon

Article

Christian et al Genes Dev. 1993 Jan. 7(1):13-28.

Insert

Xwnt-8 (wnt8a.L Frog)

Use

Xenopus expression

Tags

myc?

Expression

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only