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pRSETC-RdgBbeta-splice variant 1

Plasmid

#58276

Purpose

bacterial expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
pAR-9.47_VP1,3

Plasmid

#74240

Purpose

Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA4

Plasmid

#171002

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

Plasmid

#39858

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

IRES2-mRuby

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
Mouse GFP-PRMT7 Wild Type

Plasmid

#65056

Purpose

Expresses wild type mouse GFP-PRMT7

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

Insert

PRMT7 (Prmt7 Mouse)

Use

Bacterial propagation

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pONSY-CoH2B:Venus

Plasmid

#111877

Purpose

This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Capsaspora Histone H2B (CoH2B) fused to Venus

Use

Capsaspora owczarzaki

Tags

Venus

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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ss-ED-mCherry-GPI

Plasmid

#163680

Purpose

Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

The luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)

Tags

GPI anchor, mCherry, and signal sequences

Expression

Mammalian

Mutation

This chimera contains the ecto-domain of ST. Its …

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

Plasmid

#39859

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
DNMT2Δ191-237

Plasmid

#198382

Purpose

Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

Depositor

Tanja Schirmeister

Article

Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

Insert

DNMT2 (TRDMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

deleted amino acids 191-237

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna3.1_mouseST6galI

Plasmid

#178435

Purpose

murine St6gal1 expression plasmid

Depositor

Deborah Fass

Article

Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

Insert

St6gal1 (St6gal1 Mouse)

Tags

6xHIS-TEV Cleavage Site

Expression

Mammalian

Mutation

Coding sequnce for amino acids 85-403

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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BMP10 N67Q N131Q D4G (S2-2)

Plasmid

#184622

Purpose

for transfection into S2 cells and expression of BMP10 protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

BMP10 (BMP10 Human)

Tags

8x His - streptavidin binding peptide

Expression

Insect

Mutation

N67Q, N131Q, ARIRR(312-316) replaced with G

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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TMD20

Plasmid

#163675

Purpose

Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD21

Plasmid

#163676

Purpose

Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD22

Plasmid

#163677

Purpose

Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD24

Plasmid

#163678

Purpose

Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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F9F17F-GFP

Plasmid

#163679

Purpose

Expresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

transferrin receptor mutant (TFRC Human)

Tags

GFP

Expression

Mammalian

Mutation

This chimera contains 9 amino acids from the cyto…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA3

Plasmid

#171001

Purpose

HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIV]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA5

Plasmid

#171003

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI, R537A]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO FAPP2-GLTPH

Plasmid

#170739

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

PLEKHA8-GLTPH (PLEKHA8 Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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