We narrowed to 1,459 results for: pp

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pcDNA3.1-Gαq*-BERKY3

Plasmid

#158217

Purpose

BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-IRES-Gαq*-BERKY3

Plasmid

#158425

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gα13*-BERKY3

Plasmid

#158218

Purpose

BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY3

Plasmid

#158198

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Rho*-BERKY3

Plasmid

#158220

Purpose

BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUB-cSypHer

Plasmid

#84733

Purpose

Expresses SypHer in plant cells-biosensor for pH

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

SypHer

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHDE-35S-Cas9-mCherry-UBQ

Plasmid

#78932

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

Depositor

Yunde Zhao

Article

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-Tipin

Plasmid

#22889

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
PAGFP_NS2B3

Plasmid

#195755

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Depositor

Partha Mondal

Article

Varghese et al bioRxiv 2022.12.18.520514

Insert

Dengue (Type-2) NS2B3

Tags

PA-GFP

Expression

Mammalian

Mutation

No mutations

Promoter

CMV

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHDE-35S-Cas9-mCherry

Plasmid

#78931

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

Depositor

Yunde Zhao

Article

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ154

Plasmid

#69305

Purpose

Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9 (Arabidopsis codon optimized)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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