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pUB-sSypHer (RbcS-SypHer)
Plasmid#84732PurposeExpresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stomaDepositorInsertsSypHer
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-EPSIN 1
Plasmid#22225DepositorInsertEpsin 1 (Epn1 Rat)
UseTagsXpressExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3-MAPK-E3mut
Plasmid#29691DepositorInsertGFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK
UseTagsFlag and GFPExpressionMammalianMutationrecognition sites only, and E3 ligase site mutatedPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opto-FFR3
Plasmid#106065PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-FFR3; Opto-FFR3; F7; 718)DepositorInsertOpto-FFR3
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-PRGRH-Nluc
Plasmid#158608PurposePlasmid expressing PRG(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-p115RH-Nluc
Plasmid#158607PurposePlasmid expressing p115(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3-MAPK
Plasmid#29689PurposeGFP reporter protein fused to three GSK3 phosphorylation sitesDepositorInsertGFP glycogen synthase kinase 3 recognition site primed by MAPK
UseTagsFlag and Flag-EGFPExpressionMammalianMutationrecognition sites only, and E3 ligase sitePromoterSP6Available sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2 GFP-GSK3mut-MAPK
Plasmid#29690PurposeGFP reporter protein fused to three mutated GSK3 phosphorylation sitesDepositorInsertGFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
UseTagsFlag and Flag-EGFPExpressionMammalianMutationrecognition sites only, and E3 ligase site, GSK3 …PromoterSP6Available sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLIC-myc-GBAi W211A
Plasmid#171754PurposeFor the mammalian expression of myc-GBAi W211ADepositorInsertGBAi
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Chk1-Flag
Plasmid#22894DepositorInsertChk1 (CHEK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAF0084 Ef1a-Cp36-2a-EGFP
Plasmid#193462PurposeExpression of RecombinaseDepositorInsertCp36
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of RecombinaseDepositorInsertPa01
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0687 Pa01_attP-mCherry donor
Plasmid#193466PurposePromoterless mcherry donor for Pa01DepositorInsertPa01_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0036 Ef1a-Si74-2a-EGFP
Plasmid#193463PurposeExpression of RecombinaseDepositorInsertSi74
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0034 Ef1a-Kp03-2a-EGFP
Plasmid#193461PurposeExpression of RecombinaseDepositorInsertKp03
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF0035 Ef1a-Nm60-2a-EGFP
Plasmid#193464PurposeExpression of RecombinaseDepositorInsertNm60
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
