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KIF11 F5.3 gRNA

Plasmid

#90716

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF11

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF11 (Guide Designation F5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2294

Plasmid

#36727

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

APC-TALEN_Left (APC Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
RNASEH2A B10.3 gRNA

Plasmid

#90879

Purpose

3rd generation lentiviral gRNA plasmid targeting human RNASEH2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RNASEH2A (Guide Designation B10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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Ef1a-QCP-3xBFP

Plasmid

#158206

Purpose

Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

QCP under the Elfa promoter and three BFP tags

Use

Synthetic Biology

Tags

3xBFP

Expression

Mammalian

Promoter

Elfa

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSN22

Plasmid

#100107

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'strong' VP16 domain (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FOXM1 C12.2 gRNA

Plasmid

#90691

Purpose

3rd generation lentiviral gRNA plasmid targeting human FOXM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FOXM1 (Guide Designation C12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a in pCSDEST2

Plasmid

#140880

Purpose

Expression of zebrafish GFP-tagged Rab8a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8a (rab8a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA1_MYOD1

Plasmid

#64136

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA2_MYOD1

Plasmid

#64137

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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DHCR7 B6.3 gRNA

Plasmid

#90651

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B6.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DHCR7 B5.3 gRNA

Plasmid

#90650

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CCNA2 B2.1 gRNA

Plasmid

#90575

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNA2 (Guide Designation B2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CCNA2 B1.1 gRNA

Plasmid

#90574

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNA2 (Guide Designation B1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

Plasmid

#158989

Purpose

Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-SID4X

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-AlstR

Plasmid

#32647

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
AlstR (AstA-R1 Fly)

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
Zebrafish-gRNA-0008

Plasmid

#42248

Purpose

gRNA targeted to zebrafish gene tia1l

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-tia1l (tia1l Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
TOP2A G11.2 gRNA

Plasmid

#90914

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pST1374-Lb-Cpf1-NLS

Plasmid

#107680

Purpose

Expresses LbCpf1 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

LbCpf1

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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ANLN A2.2 gRNA

Plasmid

#90531

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANLN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANLN (Guide Designation A2.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184703

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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