We narrowed to 165,343 results for: addgene

1. PB-eDIO-ChR2-EYFP

   Plasmid

   #104387

   Purpose

   PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Channelrhodopsin 2
   

   Use

   Cre/Lox; Piggybac

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E134R

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

   Plasmid

   #52893

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[2]
   

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

3. TAL2295

   Plasmid

   #36728

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   APC-TALEN_Right (APC Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. UBE2C H5.2 gRNA

   Plasmid

   #90934

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human UBE2C

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   UBE2C (Guide Designation H5.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIS1 DICER1 long-mut-let-7

   Plasmid

   #21651

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant let-7 microRNA binding sites

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIW086

   Plasmid

   #134132

   Purpose

   Bacterial expression of His-TEV-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-TEV-human Ska1 ; human Ska2
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K183A K184A

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TUBG1 F7.4 gRNA

   Plasmid

   #90930

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TUBG1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TUBG1 (Guide Designation F7.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NUDC D9.4 gRNA

   Plasmid

   #90806

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NUDC

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NUDC (Guide Designation D9.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MIS18BP1 F9.1 gRNA

   Plasmid

   #90768

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MIS18BP1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MIS18BP1 (Guide Designation F9.1)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCNL-N1_PTS2

    Plasmid

    #65676

    Purpose

    Expresses PTS2-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)

    Plasmid

    #52906

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[2]+Venus
    

    Use

    Retroviral

    Tags

    L-IFP-F[2]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pIS1 DICER1 long-mut-all

    Plasmid

    #21653

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated let-7 and miR103/107 microRNA binding sit…

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFGC5941-PacI-ath-STTM773a/b

    Plasmid

    #84141

    Purpose

    target miRNA773a/b for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM773a/b
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCNL-N1_zyxin

    Plasmid

    #65682

    Purpose

    Expresses Zyxin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    zyxin (Zyx Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pIS1 DICER1 long-mut-miR103/107

    Plasmid

    #21652

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant miR103/107 microRNA binding sites

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PPP1CB C3.5 gRNA

    Plasmid

    #90845

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PPP1CB

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PPP1CB (Guide Designation C3.5)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ANLN A1.2 gRNA

    Plasmid

    #90530

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ANLN

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ANLN (Guide Designation A1.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgRNA3_MYOD1

    Plasmid

    #64138

    Purpose

    Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_MYOD1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AURKA A2.1 gRNA

    Plasmid

    #90541

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AURKA

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    AURKA (Guide Designation A2.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge