We narrowed to 8,342 results for: GAL

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5aa SunTag VP64 AP3

Plasmid

#115484

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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mVenus-P115-C1

Plasmid

#112931

Purpose

p115RhoGEF tagged with mVenus for detection of Ga13 activity

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

p115 RhoGEF

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA Donor_CAG

Plasmid

#229780

Purpose

Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-αC

Plasmid

#197650

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-2 αC

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Ser677T substitution

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mCherry

Plasmid

#169411

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mCherry

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc-GWS1B

Plasmid

#239582

Purpose

High copy vector for expression of GWS1B Rubisco

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

GWS1B Rubisco (6X his)

Tags

6X histag

Expression

Bacterial

Mutation

Wild-type

Promoter

Trc

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm

Plasmid

#172210

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc double blue

Plasmid

#170050

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mTagBFP2
CO BFP

Use

Unspecified

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAJM.884

Plasmid

#108536

Purpose

AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AcuRAM + PAcu-YFP

Expression

Bacterial

Mutation

AcuR: E80K R154H L202M

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHYX137

Plasmid

#202814

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX138

Plasmid

#202815

Purpose

Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Type

Empty backbone

Expression

Yeast

Promoter

ScADH2

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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UPB-pancreas (UPB7)

Plasmid

#180787

Purpose

Unique Projection Barcode (UPB7)-Pancreas for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM4D(Gi) (partial )

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm-T1T2

Plasmid

#172212

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminator

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMB34

Plasmid

#122245

Purpose

Lentivirus delivery for stable expression of LbCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pT-GEM(2)

Plasmid

#62894

Purpose

Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45564

Purpose

Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

d14 eGFP donor
I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Mutation

deleted n' 15 amino acids

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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G13 sensor (Ga13_2)

Plasmid

#112930

Purpose

A FRET sensor for G13 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

G13 sensor

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc double green

Plasmid

#169412

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mWasabi
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc triple red

Plasmid

#169413

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet proper
COmScarlet
mRuby3

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pT-GEM(1)

Plasmid

#62893

Purpose

Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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