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SuperNova-N1

Plasmid

#131853

Purpose

Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES hygro

Plasmid

#206238

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pf CAG mc2 IRES blast

Plasmid

#206235

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pf CAG mc2 IRES puro

Plasmid

#206233

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pWALIUM-intron 3x UAS attB

Plasmid

#206360

Purpose

includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

Depositor

Erdem Bangi

Article

Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAE_14-3-3

Plasmid

#109942

Purpose

Protein expression and purification of YWHAE_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAE_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pF CAG mc2 IRES hygro

Plasmid

#206234

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Article

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_SFN_14-3-3

Plasmid

#109937

Purpose

Protein expression and purification of SFN_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SFN_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc2

Plasmid

#134451

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-mCer

Plasmid

#185602

Purpose

Lentiviral construct for expression of mCerulean targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-mCer

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3

Plasmid

#21212

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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MG-155

Plasmid

#26529

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Insert

miR-155 (MIR155 Human)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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HA-NFAT4(3-407)-GFP

Plasmid

#21664

Depositor

Anjana Rao

Article

Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

Insert

NFAT4 (NFATC3 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

Amino acids 3-407

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP

Plasmid

#75148

Purpose

TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pMF1706

Plasmid

#125582

Purpose

Expresses peroxisomal roGFP2 (po-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

po-roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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