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pDEST17_A1LigN_EC

Plasmid

#22296

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

A1 domain NAD+ dependent DNA ligase N

Tags

His tag

Expression

Bacterial

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
pPgCdH3

Plasmid

#8695

Depositor

Kami Ahmad

Article

Ahmad et al J Cell Biol 2001 Apr 2;153(1):101-10.

Insert

cid promoter, H3 (His3:CG31613 Fly)

Tags

GFP

Expression

Insect

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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CTp041-dCasRx-mCherry

Plasmid

#256438

Purpose

Mammalian expression of a human codon optimized catalytically inactive CasRx

Depositor

Patrick Hsu

Article

Chandrasekaran et al Cell Syst. 2026 Feb 18;17(2):101487. doi

Insert

dCasRx-2A-mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pET-Cre-LPETG-6xHis

Plasmid

#237224

Purpose

Expresses LPETG-tagged Cre recombinase in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Cre-LPETG (cre Synthetic)

Tags

6xHis

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGM265

Plasmid

#220179

Purpose

Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSECB sgCASP9

Plasmid

#211525

Purpose

Deletes CASP9

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgBAK1

Plasmid

#211520

Purpose

Deletes BAK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgBAK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_22 [pSG5 ORI]

Plasmid

#198935

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_21 [UARS1 ORI]

Plasmid

#198934

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

UARS1 ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_19 [panARS ORI]

Plasmid

#198932

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

panARS ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_11 [pWKS1 ORI]

Plasmid

#198925

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI

Use

Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRA1EGFP

Plasmid

#193791

Purpose

Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa_SERT_EYFP

Plasmid

#198737

Purpose

Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

Depositor

Lin Tian

Article

Vargas et al Science. 2023 Feb 17;379(6633):700-706.

Insert

Serotonin transporter and yellow fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAFZifPZ431

Plasmid

#185849

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED3>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET QUEEN7mu R92A

Plasmid

#129367

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN7mu R92A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET QUEEN7mu R99A

Plasmid

#129370

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN7mu R99A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDOE11 mas:mTq2 transient

Plasmid

#124248

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Expression

Plant

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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