Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,233 results for: puc

Clear keywords
Showing: 5001 - 5020 of 5233 results

  3. pJK576

    Plasmid
    #72527
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Mutation
    synthetic gene
    Promoter
    n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72527-plasmid-map-sequence-id-132615

    Enlarge

  4. XLone-EPB41L4A-AS1 with SNORA13

    Plasmid
    #255000
    Purpose
    Express EPB41L4A-AS1 with SNORA13 located in the intron in mammalian cells
    Depositor
    Joshua Mendell
    Insert
    EPB41L4A-AS1 with SNORA13 (EPB41L4A-AS1 Human)
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    255000-plasmid-map-sequence-id-509017

    Enlarge

  5. pCBS-5738

    Plasmid
    #249324
    Purpose
    To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(DLAA)-nScCas9(D10A)
    Expression
    Yeast
    Mutation
    DarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249324-plasmid-map-sequence-id-496429

    Enlarge

  6. pCBS-5753

    Plasmid
    #249325
    Purpose
    To express the EPEC dDarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC dDarT2(DLAA)-nScCas9(D10A)
    Expression
    Yeast
    Mutation
    DarT2(G49D, M86L, R92A, E,170A, R193A) and nScCas…
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249325-plasmid-map-sequence-id-496427

    Enlarge

  7. pCHW00010

    Plasmid
    #222435
    Purpose
    Level 0, promoter module (GGAG-AATG) containing CBS4 and tomato miniDFR promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi
    Insert
    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    222435-plasmid-map-sequence-id-472307

    Enlarge

  10. qTAG-N-Solo-mStayGold-MAP4

    Plasmid
    #227296
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227296-plasmid-map-sequence-id-452068

    Enlarge

  11. qTAG-C-Solo-mStayGold-CEP192

    Plasmid
    #227289
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227289-plasmid-map-sequence-id-452061

    Enlarge

  12. pBV90

    Plasmid
    #204601
    Purpose
    Expresses OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4338955 Oryza sativa)
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Promoter
    ScADH1
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204601-plasmid-map-sequence-id-403442

    Enlarge

  13. pJQR3

    Plasmid
    #218289
    Purpose
    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218289-plasmid-map-sequence-id-433691

    Enlarge

  14. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

    Enlarge

  15. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

    Enlarge

  16. pIRGAL43C

    Plasmid
    #218278
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218278-plasmid-map-sequence-id-433680

    Enlarge

  17. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Kai Wang
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    215544-plasmid-map-sequence-id-424979

    Enlarge

Showing: 5001 - 5020 of 5233 results