We narrowed to 11,278 results for: VARS

1. pMXS-IRES-Blast SHMT2res

   Plasmid

   #106301

   Purpose

   Expresses SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Silent mutations destroy sgRNA targeting sites

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR4 PARP2 E614Q

   Plasmid

   #192545

   Purpose

   pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
   

   Use

   Gateway entry vector

   Mutation

   changed Glu 614 to Gln

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSc-His6-Sem1Sc

   Plasmid

   #83235

   Purpose

   pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Sem 1 (SEM1 Budding Yeast)
   

   Tags

   His6

   Expression

   Yeast

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PF4V1-bio-His

   Plasmid

   #52073

   Purpose

   Expresses full-length Platelet factor 4 variant precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PF4V1 (PF4V1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS2157

   Plasmid

   #70118

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-EmGFP-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1P POLE2

   Plasmid

   #160766

   Purpose

   Suppress POLE2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLE2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PV225

   Plasmid

   #132334

   Purpose

   dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
   

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe EGFR Del2(T790M)

   Plasmid

   #32075

   Depositor

   Matthew Meyerson

   Article

   Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Delete S752-I759, T790M

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pHH0103_ARHGAP4-1/1

   Plasmid

   #91518

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGAP4-1/1 (ARHGAP4 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_ARHGAP9-1/1

    Plasmid

    #91442

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP9-1/1 (ARHGAP9 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe EGFR Del4(T790M)

    Plasmid

    #32074

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Delete L747-P753, insert S, T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. sgRNA BRCA2 V572I

    Plasmid

    #139321

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 V572I using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTFP1-EB3

    Plasmid

    #55477

    Purpose

    Localization: MT End Binding Protein, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

14. mTFP1-H1-10

    Plasmid

    #55487

    Purpose

    Localization: Nucleus/Histones, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    H1 (H1f0 Mouse)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pUPD2 PhiC31 integrase (GB1496)

    Plasmid

    #160578

    Purpose

    Streptomyces phage PhiC31 integrase, complete CDS

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDual-eGFP(H66)

    Plasmid

    #63558

    Purpose

    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(H66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1P POLD1_1

    Plasmid

    #160791

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_1
    

    Use

    Lentiviral

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1P REV3L_2

    Plasmid

    #160798

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_2
    

    Use

    Lentiviral

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1P REV3L_1

    Plasmid

    #160797

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_1
    

    Use

    Lentiviral

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTFP1-LaminB1-7

    Plasmid

    #55492

    Purpose

    Localization: Nuclear Envelope, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only